Detailed information of TA system TA215876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1847784..1848374
• Replicon: chromosome
• Accession: NZ_CP083011
• Organism: Klebsiella pneumoniae strain 6710.71

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K9F28_RS09095
• Protein ID: WP_023341911.1
• Coordinates: 1848042..1848374 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: K9F28_RS09090
• Protein ID: WP_000288812.1
• Coordinates: 1847784..1848041 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F28_RS09065 (1843694)	1843694..1843768	+	75	Protein_1782	type II toxin-antitoxin system PemK/MazF family toxin	-
K9F28_RS09070 (1844119)	1844119..1845867	+	1749	WP_032418698.1	hypothetical protein	-
K9F28_RS09075 (1845921)	1845921..1846502	+	582	WP_099184326.1	hypothetical protein	-
K9F28_RS09080 (1846785)	1846785..1847246	+	462	WP_004213450.1	hypothetical protein	-
K9F28_RS09085 (1847243)	1847243..1847449	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
K9F28_RS09090 (1847784)	1847784..1848041	+	258	WP_000288812.1	antitoxin	Antitoxin
K9F28_RS09095 (1848042)	1848042..1848374	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9F28_RS09105 (1848696)	1848696..1850132	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
K9F28_RS09115 (1850498)	1850498..1851952	-	1455	WP_004148975.1	AMP nucleosidase	-
K9F28_RS09120 (1852082)	1852082..1852327	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T215876 WP_023341911.1 NZ_CP083011:1848042-1848374 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T215876 NZ_CP110988:3531752-3531970 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT215876 WP_000288812.1 NZ_CP083011:1847784-1848041 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT215876 NZ_CP110988:3531352-3531726 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0