Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 110224..110867 Replicon plasmid p1-6711.43-KPC2
Accession NZ_CP083008
Organism Klebsiella pneumoniae strain 6711.43

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F29_RS27300 Protein ID WP_001044770.1
Coordinates 110451..110867 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F29_RS27295 Protein ID WP_001261282.1
Coordinates 110224..110454 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F29_RS27260 (K9F29_27235) 105422..105895 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
K9F29_RS27265 (K9F29_27240) 107109..107891 - 783 WP_004152340.1 site-specific integrase -
K9F29_RS27270 (K9F29_27245) 107891..108223 - 333 WP_004152339.1 hypothetical protein -
K9F29_RS27275 (K9F29_27250) 108230..108628 - 399 WP_004171440.1 hypothetical protein -
K9F29_RS27280 (K9F29_27255) 108654..108983 - 330 WP_004152337.1 hypothetical protein -
K9F29_RS27285 (K9F29_27260) 109011..109319 - 309 WP_004152336.1 hypothetical protein -
K9F29_RS27290 (K9F29_27265) 109806..110267 - 462 WP_072093212.1 hypothetical protein -
K9F29_RS27295 (K9F29_27270) 110224..110454 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F29_RS27300 (K9F29_27275) 110451..110867 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F29_RS27305 (K9F29_27280) 110941..111630 + 690 Protein_127 AAA family ATPase -
K9F29_RS27310 (K9F29_27285) 111685..112382 + 698 WP_223174630.1 IS1 family transposase -
K9F29_RS27315 (K9F29_27290) 112398..112508 + 111 Protein_129 mercuric transport protein periplasmic component -
K9F29_RS27320 (K9F29_27295) 112544..112966 + 423 WP_001340589.1 organomercurial transporter MerC -
K9F29_RS27325 (K9F29_27300) 113018..114712 + 1695 WP_000105636.1 mercury(II) reductase -
K9F29_RS27330 (K9F29_27305) 114730..115092 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
K9F29_RS27335 (K9F29_27310) 115089..115325 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1A / blaOXA-9 / blaKPC-2 - 1..211349 211349
- flank IS/Tn - - 103983..105302 1319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T215873 WP_001044770.1 NZ_CP083008:110451-110867 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215873 NZ_CP110988:c2415549-2415373 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT215873 WP_001261282.1 NZ_CP083008:110224-110454 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215873 NZ_CP110988:c2415327-2414911 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References