Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5330391..5331016 Replicon chromosome
Accession NZ_CP083007
Organism Klebsiella pneumoniae strain 6711.43

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F29_RS26310 Protein ID WP_002882817.1
Coordinates 5330391..5330774 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F29_RS26315 Protein ID WP_004150355.1
Coordinates 5330774..5331016 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F29_RS26295 (K9F29_26285) 5327757..5328659 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F29_RS26300 (K9F29_26290) 5328656..5329291 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F29_RS26305 (K9F29_26295) 5329288..5330217 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F29_RS26310 (K9F29_26300) 5330391..5330774 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F29_RS26315 (K9F29_26305) 5330774..5331016 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F29_RS26320 (K9F29_26310) 5331221..5332138 + 918 WP_002882812.1 alpha/beta hydrolase -
K9F29_RS26325 (K9F29_26315) 5332152..5333093 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F29_RS26330 (K9F29_26320) 5333138..5333575 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F29_RS26335 (K9F29_26325) 5333572..5334432 - 861 WP_002882807.1 virulence factor BrkB family protein -
K9F29_RS26340 (K9F29_26330) 5334426..5335025 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215871 WP_002882817.1 NZ_CP083007:c5330774-5330391 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215871 NZ_CP110988:2011599-2011702 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215871 WP_004150355.1 NZ_CP083007:c5331016-5330774 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215871 NZ_CP110988:c2011740-2011469 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References