Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4742839..4743649 Replicon chromosome
Accession NZ_CP083007
Organism Klebsiella pneumoniae strain 6711.43

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K9F29_RS23495 Protein ID WP_002887280.1
Coordinates 4742839..4743372 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F29_RS23500 Protein ID WP_002887278.1
Coordinates 4743383..4743649 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F29_RS23490 (K9F29_23480) 4741670..4742791 + 1122 WP_002887282.1 cupin domain-containing protein -
K9F29_RS23495 (K9F29_23485) 4742839..4743372 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K9F29_RS23500 (K9F29_23490) 4743383..4743649 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F29_RS23505 (K9F29_23495) 4743752..4745185 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F29_RS23510 (K9F29_23500) 4745175..4745858 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K9F29_RS23515 (K9F29_23505) 4746030..4747415 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
K9F29_RS23520 (K9F29_23510) 4747433..4747777 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T215868 WP_002887280.1 NZ_CP083007:c4743372-4742839 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215868 NZ_CP110988:1805649-1805900 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215868 WP_002887278.1 NZ_CP083007:c4743649-4743383 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215868 NZ_CP110988:1805897-1806151 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References