Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4130167..4130786 Replicon chromosome
Accession NZ_CP083007
Organism Klebsiella pneumoniae strain 6711.43

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K9F29_RS20550 Protein ID WP_002892050.1
Coordinates 4130568..4130786 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K9F29_RS20545 Protein ID WP_002892066.1
Coordinates 4130167..4130541 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F29_RS20535 (K9F29_20525) 4125319..4126512 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K9F29_RS20540 (K9F29_20530) 4126535..4129681 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K9F29_RS20545 (K9F29_20535) 4130167..4130541 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K9F29_RS20550 (K9F29_20540) 4130568..4130786 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K9F29_RS20555 (K9F29_20545) 4130945..4131511 + 567 WP_002892030.1 maltose O-acetyltransferase -
K9F29_RS20560 (K9F29_20550) 4131483..4131623 - 141 WP_004147370.1 hypothetical protein -
K9F29_RS20565 (K9F29_20555) 4131644..4132114 + 471 WP_002892026.1 YlaC family protein -
K9F29_RS20570 (K9F29_20560) 4132089..4133540 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K9F29_RS20575 (K9F29_20565) 4133641..4134339 + 699 WP_002892021.1 GNAT family protein -
K9F29_RS20580 (K9F29_20570) 4134336..4134476 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K9F29_RS20585 (K9F29_20575) 4134476..4134739 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215867 WP_002892050.1 NZ_CP083007:4130568-4130786 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215867 NZ_CP110988:1032170-1032505 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGATTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215867 WP_002892066.1 NZ_CP083007:4130167-4130541 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215867 NZ_CP110988:1031922-1032170 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References