Detailed information of TA system TA215853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1683..2157
• Replicon: plasmid p4-6712.08
• Accession: NZ_CP083004
• Organism: Klebsiella pneumoniae strain 6712.08

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: K9F30_RS27870
• Protein ID: WP_004197762.1
• Coordinates: 1891..2157 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: K9F30_RS27865
• Protein ID: WP_000879771.1
• Coordinates: 1683..1907 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F30_RS27850	1..5598	-	5598	WP_032433971.1	hypothetical protein	-
K9F30_RS27855 (K9F30_27810)	288..797	+	510	WP_032433972.1	MbeB family mobilization protein	-
K9F30_RS27860 (K9F30_27815)	804..1016	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
K9F30_RS28005	1392..1520	-	129	WP_000523570.1	hypothetical protein	-
K9F30_RS27865 (K9F30_27820)	1683..1907	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
K9F30_RS27870 (K9F30_27825)	1891..2157	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9F30_RS27875 (K9F30_27830)	2227..2577	-	351	WP_004197756.1	hypothetical protein	-
K9F30_RS27880 (K9F30_27835)	2643..3188	-	546	WP_032433973.1	hypothetical protein	-
K9F30_RS27885 (K9F30_27840)	3214..3540	-	327	WP_004197760.1	hypothetical protein	-
K9F30_RS27890 (K9F30_27845)	4698..4874	+	177	WP_165764108.1	hypothetical protein	-
K9F30_RS27895 (K9F30_27850)	4864..5208	+	345	WP_004197764.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5598	5598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T215853 WP_004197762.1 NZ_CP083004:1891-2157 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T215853 NZ_CP110978:c3649921-3649523 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT215853 WP_000879771.1 NZ_CP083004:1683-1907 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT215853 NZ_CP110978:c3650217-3649924 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5