Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19348..19991 Replicon plasmid p1-6712.08-KPC3
Accession NZ_CP083001
Organism Klebsiella pneumoniae strain 6712.08

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F30_RS26825 Protein ID WP_001044770.1
Coordinates 19575..19991 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F30_RS26820 Protein ID WP_001261282.1
Coordinates 19348..19578 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F30_RS26785 (K9F30_26745) 14920..15786 - 867 WP_004118283.1 replication initiation protein -
K9F30_RS26790 (K9F30_26750) 16321..16425 + 105 WP_032409716.1 hypothetical protein -
K9F30_RS26795 (K9F30_26755) 16554..16811 + 258 WP_000764642.1 hypothetical protein -
K9F30_RS26800 (K9F30_26760) 16869..17645 - 777 WP_000015958.1 site-specific integrase -
K9F30_RS26805 (K9F30_26765) 17642..18385 - 744 WP_000129823.1 hypothetical protein -
K9F30_RS26810 (K9F30_26770) 18436..18786 - 351 WP_000493378.1 hypothetical protein -
K9F30_RS26815 (K9F30_26775) 18930..19391 - 462 WP_072202616.1 hypothetical protein -
K9F30_RS26820 (K9F30_26780) 19348..19578 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F30_RS26825 (K9F30_26785) 19575..19991 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F30_RS26830 (K9F30_26790) 20065..20754 + 690 Protein_27 AAA family ATPase -
K9F30_RS26835 (K9F30_26795) 20809..21506 + 698 WP_223174630.1 IS1 family transposase -
K9F30_RS26840 (K9F30_26800) 21522..21632 + 111 Protein_29 mercuric transport protein periplasmic component -
K9F30_RS26845 (K9F30_26805) 21668..22090 + 423 WP_001340589.1 organomercurial transporter MerC -
K9F30_RS26850 (K9F30_26810) 22142..23836 + 1695 WP_000105636.1 mercury(II) reductase -
K9F30_RS26855 (K9F30_26815) 23854..24216 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
K9F30_RS26860 (K9F30_26820) 24213..24449 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / blaOXA-9 / mph(E) / msr(E) / armA / blaKPC-3 - 1..140975 140975


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T215852 WP_001044770.1 NZ_CP083001:19575-19991 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215852 NZ_CP110978:3475501-3476043 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGCTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT215852 WP_001261282.1 NZ_CP083001:19348-19578 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215852 NZ_CP110978:3475206-3475517 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References