Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5373975..5374600 Replicon chromosome
Accession NZ_CP083000
Organism Klebsiella pneumoniae strain 6712.08

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F30_RS26330 Protein ID WP_002882817.1
Coordinates 5373975..5374358 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F30_RS26335 Protein ID WP_004150355.1
Coordinates 5374358..5374600 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F30_RS26315 (5371341) 5371341..5372243 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F30_RS26320 (5372240) 5372240..5372875 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F30_RS26325 (5372872) 5372872..5373801 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F30_RS26330 (5373975) 5373975..5374358 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F30_RS26335 (5374358) 5374358..5374600 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F30_RS26340 (5374805) 5374805..5375722 + 918 WP_032418237.1 alpha/beta hydrolase -
K9F30_RS26345 (5375736) 5375736..5376677 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F30_RS26350 (5376722) 5376722..5377159 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F30_RS26355 (5377156) 5377156..5378016 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F30_RS26360 (5378010) 5378010..5378609 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215851 WP_002882817.1 NZ_CP083000:c5374358-5373975 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215851 NZ_CP110978:3441527-3441745 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215851 WP_004150355.1 NZ_CP083000:c5374600-5374358 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215851 NZ_CP110978:3441127-3441501 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References