Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4775374..4776184 Replicon chromosome
Accession NZ_CP083000
Organism Klebsiella pneumoniae strain 6712.08

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K9F30_RS23510 Protein ID WP_004178461.1
Coordinates 4775374..4775907 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F30_RS23515 Protein ID WP_002887278.1
Coordinates 4775918..4776184 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F30_RS23505 (4774205) 4774205..4775326 + 1122 WP_004214138.1 cupin domain-containing protein -
K9F30_RS23510 (4775374) 4775374..4775907 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K9F30_RS23515 (4775918) 4775918..4776184 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F30_RS23520 (4776287) 4776287..4777720 - 1434 Protein_4623 Cu(+)/Ag(+) sensor histidine kinase -
K9F30_RS23525 (4777710) 4777710..4778393 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K9F30_RS23530 (4778565) 4778565..4779950 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
K9F30_RS23535 (4779968) 4779968..4780312 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T215849 WP_004178461.1 NZ_CP083000:c4775907-4775374 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215849 NZ_CP110978:2645370-2645477 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215849 WP_002887278.1 NZ_CP083000:c4776184-4775918 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215849 NZ_CP110978:c2645320-2645257 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References