Detailed information of TA system TA215837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1848447..1849037
• Replicon: chromosome
• Accession: NZ_CP083000
• Organism: Klebsiella pneumoniae strain 6712.08

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K9F30_RS09090
• Protein ID: WP_023341911.1
• Coordinates: 1848705..1849037 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: K9F30_RS09085
• Protein ID: WP_000288812.1
• Coordinates: 1848447..1848704 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F30_RS09060 (1844357)	1844357..1844431	+	75	Protein_1780	type II toxin-antitoxin system PemK/MazF family toxin	-
K9F30_RS09065 (1844782)	1844782..1846530	+	1749	WP_032418698.1	hypothetical protein	-
K9F30_RS09070 (1846584)	1846584..1847165	+	582	WP_099184326.1	hypothetical protein	-
K9F30_RS09075 (1847448)	1847448..1847909	+	462	WP_004213450.1	hypothetical protein	-
K9F30_RS09080 (1847906)	1847906..1848112	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
K9F30_RS09085 (1848447)	1848447..1848704	+	258	WP_000288812.1	antitoxin	Antitoxin
K9F30_RS09090 (1848705)	1848705..1849037	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9F30_RS09100 (1849359)	1849359..1850795	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
K9F30_RS09110 (1851161)	1851161..1852615	-	1455	WP_004148975.1	AMP nucleosidase	-
K9F30_RS09115 (1852745)	1852745..1852990	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T215837 WP_023341911.1 NZ_CP083000:1848705-1849037 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T215837 NZ_CP110978:c49999-49622 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCTGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT215837 WP_000288812.1 NZ_CP083000:1848447-1848704 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT215837 NZ_CP110978:c50457-50089 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATACATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0