Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5250097..5250722 Replicon chromosome
Accession NZ_CP082995
Organism Klebsiella pneumoniae strain 6911.28

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F31_RS25885 Protein ID WP_002882817.1
Coordinates 5250097..5250480 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F31_RS25890 Protein ID WP_004150355.1
Coordinates 5250480..5250722 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F31_RS25870 (K9F31_25835) 5247463..5248365 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F31_RS25875 (K9F31_25840) 5248362..5248997 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F31_RS25880 (K9F31_25845) 5248994..5249923 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F31_RS25885 (K9F31_25850) 5250097..5250480 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F31_RS25890 (K9F31_25855) 5250480..5250722 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F31_RS25895 (K9F31_25860) 5250927..5251844 + 918 WP_002882812.1 alpha/beta hydrolase -
K9F31_RS25900 (K9F31_25865) 5251858..5252799 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F31_RS25905 (K9F31_25870) 5252844..5253281 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F31_RS25910 (K9F31_25875) 5253278..5254138 - 861 WP_002882807.1 virulence factor BrkB family protein -
K9F31_RS25915 (K9F31_25880) 5254132..5254731 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215833 WP_002882817.1 NZ_CP082995:c5250480-5250097 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215833 NZ_CP110969:c14266-13916 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215833 WP_004150355.1 NZ_CP082995:c5250722-5250480 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215833 NZ_CP110969:c13911-13609 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References