Detailed information of TA system TA021583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5547015..5547631
• Replicon: chromosome
• Accession: NC_008027
• Organism: Pseudomonas entomophila L48

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q1I3D1
• Locus tag: PSEEN_RS24190
• Protein ID: WP_011536214.1
• Coordinates: 5547440..5547631 (-)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q1I3D2
• Locus tag: PSEEN_RS24185
• Protein ID: WP_011536213.1
• Coordinates: 5547015..5547422 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEEN_RS24160	5542120..5543139	-	1020	WP_011536207.1	DUF262 domain-containing protein	-
PSEEN_RS26790	5543148..5543708	-	561	WP_011536208.1	hypothetical protein	-
PSEEN_RS24165	5544272..5544487	-	216	WP_044488541.1	hypothetical protein	-
PSEEN_RS24170	5544692..5545834	+	1143	WP_011536210.1	nitrite reductase, copper-containing	-
PSEEN_RS24175	5545913..5546059	+	147	Protein_4782	DUF3079 domain-containing protein	-
PSEEN_RS24180	5546174..5546995	+	822	WP_011536212.1	suppressor of fused domain protein	-
PSEEN_RS24185	5547015..5547422	-	408	WP_011536213.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PSEEN_RS24190	5547440..5547631	-	192	WP_011536214.1	type II toxin-antitoxin system HicA family toxin	Toxin
PSEEN_RS24195	5547850..5548719	-	870	WP_011536215.1	LysR family transcriptional regulator	-
PSEEN_RS24200	5548732..5550978	-	2247	WP_011536216.1	heavy metal translocating P-type ATPase	-
PSEEN_RS24205	5551166..5551609	+	444	WP_011536217.1	cadmium resistance transcriptional regulator CadR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5539035..5548719	9684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7041.18 Da
• Isoelectric Point: 10.7339

>T21583 WP_011536214.1 NC_008027:c5547631-5547440 [Pseudomonas entomophila L48]
MNSREMIAVIEADGWYLVRSKGSHHHFKHPHKTGLVTIPHPKKDLLPATAASILRQARIGYAS

Nucleotide

• Length: 192 bp

>T21583 NC_008027:c5547631-5547440 [Pseudomonas entomophila L48]
ATGAACAGCCGCGAAATGATTGCAGTAATCGAGGCGGATGGTTGGTACCTGGTGCGTTCGAAAGGCAGCCACCACCACTT
CAAGCACCCCCACAAGACCGGACTGGTCACCATCCCGCACCCGAAGAAGGACCTGTTGCCCGCCACCGCGGCCAGCATCC
TGCGCCAGGCGCGGATCGGTTACGCCAGTTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14659.69 Da
• Isoelectric Point: 4.6025

>AT21583 WP_011536213.1 NC_008027:c5547422-5547015 [Pseudomonas entomophila L48]
MLFPIAILPGDDQHAWGVEVPDIPGCFSAGDDLDDAIAMAREAIEGHLELLAQDQQEIPKAGKVSEHAANPAFAGCTWAL
IDIDITRYLGKAEKLNITLPAYLLTRIDSYVQNHPEHKSRSGFLAEAALKVLQGK

Nucleotide

• Length: 408 bp

>AT21583 NC_008027:c5547422-5547015 [Pseudomonas entomophila L48]
ATGCTTTTCCCTATCGCGATCCTCCCCGGCGACGACCAGCACGCCTGGGGCGTGGAAGTGCCGGACATCCCTGGGTGCTT
CTCGGCCGGCGACGACCTTGACGATGCCATTGCCATGGCCCGCGAGGCGATCGAAGGCCACCTGGAATTGCTCGCCCAGG
ACCAGCAGGAGATCCCCAAGGCCGGCAAGGTCAGCGAGCATGCCGCCAACCCGGCGTTCGCCGGATGCACCTGGGCGCTG
ATCGACATCGACATCACCCGTTACCTGGGCAAGGCCGAAAAACTCAACATCACCTTGCCGGCCTACCTGCTCACCCGCAT
CGACAGCTATGTGCAGAACCACCCCGAGCACAAGAGCCGCTCGGGTTTCCTGGCCGAAGCGGCGCTCAAGGTGCTGCAGG
GCAAGTAA

Structures

Toxin

• AlphaFold DB: Q1I3D1

Antitoxin

• AlphaFold DB: Q1I3D2