Detailed information of TA system TA021582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5184967..5185607
• Replicon: chromosome
• Accession: NC_008027
• Organism: Pseudomonas entomophila L48

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PSEEN_RS22560
• Protein ID: WP_011535890.1
• Coordinates: 5185197..5185607 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: PSEEN_RS22555
• Protein ID: WP_011535889.1
• Coordinates: 5184967..5185197 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEEN_RS22535	5180319..5181644	-	1326	WP_011535885.1	precorrin-3B synthase	-
PSEEN_RS22540	5181861..5183072	+	1212	WP_011535886.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
PSEEN_RS22545	5183065..5184159	+	1095	WP_011535887.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
PSEEN_RS22550	5184156..5184884	+	729	WP_011535888.1	cobalt-precorrin-6A reductase	-
PSEEN_RS22555	5184967..5185197	+	231	WP_011535889.1	antitoxin	Antitoxin
PSEEN_RS22560	5185197..5185607	+	411	WP_011535890.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PSEEN_RS22565	5185681..5186118	+	438	WP_011535891.1	NfeD family protein	-
PSEEN_RS22570	5186146..5187000	+	855	WP_011535892.1	SPFH/Band 7/PHB domain protein	-
PSEEN_RS22575	5187258..5187647	+	390	WP_011535893.1	DUF2946 domain-containing protein	-
PSEEN_RS22580	5187695..5188177	+	483	WP_011535894.1	copper chaperone PCu(A)C	-
PSEEN_RS22585	5188202..5188633	+	432	WP_011535895.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15210.56 Da
• Isoelectric Point: 9.4576

>T21582 WP_011535890.1 NC_008027:5185197-5185607 [Pseudomonas entomophila L48]
MLRYMLDTNICIFTIKNKPQVVREAFNRYHGQLAISTVTLMELIYGAEKSAAPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTSPR

Nucleotide

• Length: 411 bp

>T21582 NC_008027:5185197-5185607 [Pseudomonas entomophila L48]
ATGCTCAGGTACATGCTCGATACCAACATCTGCATTTTCACCATCAAGAACAAGCCACAGGTAGTGCGCGAGGCGTTCAA
TCGCTACCACGGCCAGCTGGCCATCAGCACAGTAACCCTGATGGAGTTGATCTACGGTGCGGAAAAATCCGCCGCGCCGG
AACGTAACCTGGCCATTGTCGAGGGGTTCGCCGCACGGCTCGATGTGCTTGACTACGACATTCAGGGCGCCGCTCACACT
GCGCAGCTACGTGCGGAGCTGGCCAAGGCCGGCACGCCCATCGGCCCCTACGACAGAATGATTGCCGGCCACGCCCGCGC
TCGCGGCCTCACACTGGTGACCAACAACCTGCGCGAGTTCCAGCGTGTTCCGGGGTTGCGTGTGGAAGACTGGGTGACGT
CCCCACGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT21582 WP_011535889.1 NC_008027:5184967-5185197 [Pseudomonas entomophila L48]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT21582 NC_008027:5184967-5185197 [Pseudomonas entomophila L48]
ATGGAGCAAGGTGCAGTCTTCAAAAGCAACCGCAGCCAGGCTATCCGCATGCCAAAAGCCCTCGCCCTTCCAGAAGACGT
CACCCGCGTGGACATCGTCGCGGTGGGCCGTTCCCGTATCATCTCGCCTGCCGGCGAAAGCTGGGACAGCTGGTTCGAGG
GTGACGATGTGAGTGCGGACTTCATGGCGAGCAGGGAACAGCCAGCCGACCAGGAGCGCGAAGGATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6