Detailed information of TA system TA021580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-DUF971
• Location: 4233170..4233770
• Replicon: chromosome
• Accession: NC_008027
• Organism: Pseudomonas entomophila L48

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q1I6T8
• Locus tag: PSEEN_RS18185
• Protein ID: WP_011535019.1
• Coordinates: 4233170..4233472 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PSEEN_RS18190
• Protein ID: WP_011535020.1
• Coordinates: 4233465..4233770 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEEN_RS18170	4229179..4230558	+	1380	WP_011535015.1	sodium:solute symporter	-
PSEEN_RS18175	4230926..4231819	-	894	WP_011535017.1	LysR family transcriptional regulator	-
PSEEN_RS18180	4231926..4233092	+	1167	WP_011535018.1	YbfB/YjiJ family MFS transporter	-
PSEEN_RS18185	4233170..4233472	+	303	WP_011535019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSEEN_RS18190	4233465..4233770	+	306	WP_011535020.1	putative addiction module antidote protein	Antitoxin
PSEEN_RS18195	4233844..4234770	-	927	WP_011535021.1	DMT family transporter	-
PSEEN_RS18200	4235162..4235617	-	456	WP_083789191.1	DDE-type integrase/transposase/recombinase	-
PSEEN_RS18210	4235630..4236780	+	1151	WP_086009448.1	IS3-like element ISPen2 family transposase	-
PSEEN_RS26710	4236904..4237293	-	390	WP_158020264.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-90)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11329.04 Da
• Isoelectric Point: 10.6106

>T21580 WP_011535019.1 NC_008027:4233170-4233472 [Pseudomonas entomophila L48]
MKTIESSSFRRWLIGLRDTTARARIVARINRLKEGLAGDVSPVGHGMSELRVHYGPGYRVYFHQCGDTLIILLCGGDKGS
QQRDIKAARQILQSWRMQND

Nucleotide

• Length: 303 bp

>T21580 NC_008027:4233170-4233472 [Pseudomonas entomophila L48]
ATGAAAACCATCGAATCCAGCAGTTTCAGGCGTTGGCTGATCGGGCTTCGGGATACCACGGCAAGGGCCAGAATCGTCGC
TCGAATCAACCGGCTCAAGGAAGGGCTGGCGGGTGACGTTTCACCGGTCGGCCATGGCATGAGTGAGCTGAGAGTCCATT
ATGGCCCAGGCTATCGGGTGTACTTTCACCAATGCGGCGATACCTTGATCATCCTGCTTTGCGGCGGCGACAAAGGCAGC
CAGCAGCGGGACATCAAGGCAGCCCGGCAGATCCTGCAGTCGTGGAGGATGCAAAATGACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10851.36 Da
• Isoelectric Point: 5.5900

>AT21580 WP_011535020.1 NC_008027:4233465-4233770 [Pseudomonas entomophila L48]
MTETFHEFDAAAYLTSPEAIAQFMTDALETGDAAYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMAARPHLVQQGVG

Nucleotide

• Length: 306 bp

>AT21580 NC_008027:4233465-4233770 [Pseudomonas entomophila L48]
ATGACTGAAACGTTCCATGAGTTCGACGCCGCCGCCTATCTGACTTCCCCGGAAGCTATCGCGCAGTTCATGACCGATGC
ATTGGAGACAGGGGATGCAGCCTACGTGGCAAAAGCCGTAGGCGTGGTAGCTCGCGCCAAGGGCATGAGTGAACTGGCAA
AGGAAACAGGCCTGTCTCGCGAACAGCTTTACCGCTCGTTCAGTGAACACGGCAATCCAACGCTCAAGTCATTCCTGACT
GTAATGAAAGCGCTGGGAATTGACATGGCTGCCAGACCTCACCTGGTCCAGCAAGGCGTGGGCTGA

Structures

Toxin

• AlphaFold DB: Q1I6T8