Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1468793..1469529 Replicon chromosome
Accession NZ_CP082988
Organism Klebsiella pneumoniae strain 7008.20

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag K9F32_RS07255 Protein ID WP_032433360.1
Coordinates 1469047..1469529 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K9F32_RS07250 Protein ID WP_003026799.1
Coordinates 1468793..1469059 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F32_RS07225 (1464439) 1464439..1465578 + 1140 WP_032433368.1 mannitol dehydrogenase -
K9F32_RS07230 (1465607) 1465607..1466269 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
K9F32_RS07235 (1466253) 1466253..1467257 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
K9F32_RS07240 (1467275) 1467275..1467907 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
K9F32_RS07245 (1467917) 1467917..1468480 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
K9F32_RS07250 (1468793) 1468793..1469059 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K9F32_RS07255 (1469047) 1469047..1469529 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
K9F32_RS07260 (1469729) 1469729..1471132 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
K9F32_RS07265 (1471161) 1471161..1471793 - 633 WP_001567369.1 hypothetical protein -
K9F32_RS07270 (1472173) 1472173..1472772 - 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
K9F32_RS07275 (1472985) 1472985..1473929 - 945 WP_077254249.1 fimbrial protein -
K9F32_RS07280 (1473941) 1473941..1474519 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1436738..1482755 46017
- flank IS/Tn - - 1469729..1471132 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T215798 WP_032433360.1 NZ_CP082988:1469047-1469529 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T215798 NZ_CP110957:740107-740592 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT215798 WP_003026799.1 NZ_CP082988:1468793-1469059 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT215798 NZ_CP110957:739817-740110 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References