Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1452102..1452745 Replicon chromosome
Accession NZ_CP082988
Organism Klebsiella pneumoniae strain 7008.20

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag K9F32_RS07160 Protein ID WP_032433387.1
Coordinates 1452329..1452745 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag K9F32_RS07155 Protein ID WP_001261276.1
Coordinates 1452102..1452332 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F32_RS07140 (1447124) 1447124..1448211 + 1088 Protein_1395 transcriptional regulator -
K9F32_RS07145 (1448214) 1448214..1450454 + 2241 WP_045326788.1 P-loop NTPase fold protein -
K9F32_RS07150 (1450982) 1450982..1451797 - 816 WP_032433388.1 hypothetical protein -
K9F32_RS07155 (1452102) 1452102..1452332 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F32_RS07160 (1452329) 1452329..1452745 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F32_RS07165 (1452901) 1452901..1453881 + 981 WP_032433385.1 hypothetical protein -
K9F32_RS07170 (1454076) 1454076..1455647 - 1572 WP_032433383.1 AAA family ATPase -
K9F32_RS07175 (1455966) 1455966..1456214 + 249 WP_032433382.1 hypothetical protein -
K9F32_RS07180 (1456273) 1456273..1456791 + 519 WP_045326794.1 hypothetical protein -
K9F32_RS07185 (1456822) 1456822..1457313 + 492 WP_032433378.1 hypothetical protein -
K9F32_RS07190 (1457373) 1457373..1457576 + 204 WP_032433376.1 HHA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1436738..1482755 46017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T215797 WP_032433387.1 NZ_CP082988:1452329-1452745 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T215797 NZ_CP110957:352722-353069 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT215797 WP_001261276.1 NZ_CP082988:1452102-1452332 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT215797 NZ_CP110957:353069-353368 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References