Detailed information of TA system TA215793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 252703..253225
• Replicon: plasmid p1-7011.62-NDM1
• Accession: NZ_CP082986
• Organism: Klebsiella pneumoniae strain 7011.62

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: K9F33_RS27315
• Protein ID: WP_114267963.1
• Coordinates: 252941..253225 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: K9F33_RS27310
• Protein ID: WP_004181777.1
• Coordinates: 252703..252951 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F33_RS27280 (K9F33_27215)	248737..248901	-	165	WP_004026392.1	hypothetical protein	-
K9F33_RS27285 (K9F33_27220)	248962..249417	-	456	WP_014386579.1	DUF957 domain-containing protein	-
K9F33_RS27290 (K9F33_27225)	249705..250313	-	609	WP_004026394.1	hypothetical protein	-
K9F33_RS27295 (K9F33_27230)	250383..250832	-	450	WP_004026395.1	hypothetical protein	-
K9F33_RS27300 (K9F33_27235)	251302..251505	-	204	Protein_277	DUF550 domain-containing protein	-
K9F33_RS27305 (K9F33_27240)	251562..252530	-	969	WP_074449071.1	IS5 family transposase	-
K9F33_RS27310 (K9F33_27245)	252703..252951	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K9F33_RS27315 (K9F33_27250)	252941..253225	+	285	WP_114267963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9F33_RS27320 (K9F33_27255)	253242..253343	-	102	Protein_281	IS200/IS605 family transposase	-
K9F33_RS27325 (K9F33_27260)	253379..254602	+	1224	WP_004181779.1	RNA-guided endonuclease TnpB family protein	-
K9F33_RS27330 (K9F33_27265)	254993..256180	-	1188	WP_004181780.1	RNA-guided endonuclease TnpB family protein	-
K9F33_RS27335 (K9F33_27270)	256846..257313	+	468	WP_124737156.1	hypothetical protein	-
K9F33_RS27340 (K9F33_27275)	257358..257564	-	207	WP_063938351.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / qnrS1 / aph(3')-VI / blaNDM-1 / catA1	-	1..342478	342478
	-	inside	IScluster/Tn	-	-	251562..256231	4669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10956.62 Da
• Isoelectric Point: 10.5816

>T215793 WP_114267963.1 NZ_CP082986:252941-253225 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYNIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T215793 NZ_CP110952:c5252037-5251654 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT215793 WP_004181777.1 NZ_CP082986:252703-252951 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT215793 NZ_CP110952:c5252279-5252037 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4R0U8