Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 55796..56582 | Replicon | plasmid p1-7011.62-NDM1 |
| Accession | NZ_CP082986 | ||
| Organism | Klebsiella pneumoniae strain 7011.62 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | R8WN26 |
| Locus tag | K9F33_RS26265 | Protein ID | WP_016154457.1 |
| Coordinates | 55796..56173 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A242VSK1 |
| Locus tag | K9F33_RS26270 | Protein ID | WP_009652436.1 |
| Coordinates | 56223..56582 (-) | Length | 120 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K9F33_RS26230 (K9F33_26170) | 51054..52022 | - | 969 | WP_074449071.1 | IS5 family transposase | - |
| K9F33_RS26235 (K9F33_26175) | 52054..52122 | - | 69 | Protein_64 | tellurium resistance protein TerY | - |
| K9F33_RS26240 (K9F33_26180) | 52442..52915 | - | 474 | WP_009652494.1 | ribbon-helix-helix protein, CopG family | - |
| K9F33_RS26245 (K9F33_26185) | 52918..53949 | - | 1032 | WP_223175620.1 | hypothetical protein | - |
| K9F33_RS26250 (K9F33_26190) | 54149..54994 | - | 846 | Protein_67 | DUF4942 domain-containing protein | - |
| K9F33_RS26255 (K9F33_26195) | 55075..55278 | - | 204 | WP_009652505.1 | DUF957 domain-containing protein | - |
| K9F33_RS26260 (K9F33_26200) | 55308..55799 | - | 492 | WP_009652438.1 | DUF5983 family protein | - |
| K9F33_RS26265 (K9F33_26205) | 55796..56173 | - | 378 | WP_016154457.1 | TA system toxin CbtA family protein | Toxin |
| K9F33_RS26270 (K9F33_26210) | 56223..56582 | - | 360 | WP_009652436.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| K9F33_RS26275 (K9F33_26215) | 56606..56827 | - | 222 | WP_009652392.1 | DUF987 domain-containing protein | - |
| K9F33_RS26280 (K9F33_26220) | 56841..57323 | - | 483 | WP_052238324.1 | DNA repair protein RadC | - |
| K9F33_RS26285 (K9F33_26225) | 57335..57553 | - | 219 | WP_009652503.1 | hypothetical protein | - |
| K9F33_RS26290 (K9F33_26230) | 57564..58037 | - | 474 | WP_009652453.1 | antirestriction protein | - |
| K9F33_RS26295 (K9F33_26235) | 58054..58308 | - | 255 | WP_009652519.1 | hypothetical protein | - |
| K9F33_RS26300 (K9F33_26240) | 58308..59126 | - | 819 | WP_009652424.1 | DUF932 domain-containing protein | - |
| K9F33_RS26305 (K9F33_26245) | 59230..59463 | - | 234 | WP_009652474.1 | DUF905 domain-containing protein | - |
| K9F33_RS26310 (K9F33_26250) | 59519..59740 | - | 222 | WP_009652443.1 | hypothetical protein | - |
| K9F33_RS26315 (K9F33_26255) | 59823..60335 | - | 513 | WP_009652395.1 | DUF4234 domain-containing protein | - |
| K9F33_RS26320 (K9F33_26260) | 60384..60854 | - | 471 | WP_009652431.1 | hypothetical protein | - |
| K9F33_RS26325 (K9F33_26265) | 60901..61212 | - | 312 | WP_009652410.1 | hypothetical protein | - |
| K9F33_RS26330 (K9F33_26270) | 61225..61491 | - | 267 | WP_009652486.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / qnrS1 / aph(3')-VI / blaNDM-1 / catA1 | - | 1..342478 | 342478 | |
| - | flank | IS/Tn | - | - | 51054..52022 | 968 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14168.19 Da Isoelectric Point: 9.5224
>T215791 WP_016154457.1 NZ_CP082986:c56173-55796 [Klebsiella pneumoniae]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP
Download Length: 378 bp
>T215791 NZ_CP110952:c4760718-4760434 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA
Antitoxin
Download Length: 120 a.a. Molecular weight: 13286.19 Da Isoelectric Point: 6.4780
>AT215791 WP_009652436.1 NZ_CP082986:c56582-56223 [Klebsiella pneumoniae]
MSNTIPPVNHDIAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELN
PRHQHCVTLYAKGLICEADSLGSHGYVYLAIYPTPATTE
MSNTIPPVNHDIAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELN
PRHQHCVTLYAKGLICEADSLGSHGYVYLAIYPTPATTE
Download Length: 360 bp
>AT215791 NZ_CP110952:c4760950-4760708 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R8WN26 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A242VSK1 |