Detailed information of TA system TA215790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5231503..5232128
• Replicon: chromosome
• Accession: NZ_CP082985
• Organism: Klebsiella pneumoniae strain 7011.62

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: K9F33_RS25555
• Protein ID: WP_002882817.1
• Coordinates: 5231503..5231886 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K9F33_RS25560
• Protein ID: WP_162557435.1
• Coordinates: 5231886..5232128 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F33_RS25540 (5228869)	5228869..5229771	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
K9F33_RS25545 (5229768)	5229768..5230403	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
K9F33_RS25550 (5230400)	5230400..5231329	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
K9F33_RS25555 (5231503)	5231503..5231886	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9F33_RS25560 (5231886)	5231886..5232128	-	243	WP_162557435.1	CopG family transcriptional regulator	Antitoxin
K9F33_RS25565 (5232333)	5232333..5233250	+	918	WP_032417884.1	alpha/beta hydrolase	-
K9F33_RS25570 (5233265)	5233265..5234206	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
K9F33_RS25575 (5234251)	5234251..5234688	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
K9F33_RS25580 (5234685)	5234685..5235545	-	861	WP_004146232.1	virulence factor BrkB family protein	-
K9F33_RS25585 (5235539)	5235539..5236138	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T215790 WP_002882817.1 NZ_CP082985:c5231886-5231503 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T215790 NZ_CP110952:c4658874-4658341 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9557.79 Da
• Isoelectric Point: 4.3509

>AT215790 WP_162557435.1 NZ_CP082985:c5232128-5231886 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNISRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT215790 NZ_CP110952:c4659151-4658885 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8