Detailed information of TA system TA021578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 292895..294624
• Replicon: chromosome
• Accession: NC_008027
• Organism: Pseudomonas entomophila L48

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: Q1IGF9
• Locus tag: PSEEN_RS01270
• Protein ID: WP_011531703.1
• Coordinates: 292895..293536 (+)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: Q1IGF8
• Locus tag: PSEEN_RS01275
• Protein ID: WP_011531704.1
• Coordinates: 293536..294624 (+)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEEN_RS01255	289532..290923	-	1392	WP_011531700.1	L-cystine transporter	-
PSEEN_RS01260	291171..292226	+	1056	WP_162042905.1	haloacid dehalogenase-like hydrolase	-
PSEEN_RS01265	292317..292898	+	582	WP_011531702.1	DUF4433 domain-containing protein	-
PSEEN_RS01270	292895..293536	+	642	WP_011531703.1	DUF4433 domain-containing protein	Toxin
PSEEN_RS01275	293536..294624	+	1089	WP_011531704.1	macro domain-containing protein	Antitoxin
PSEEN_RS01280	294683..296524	-	1842	WP_011531705.1	dihydroxy-acid dehydratase	-
PSEEN_RS01285	296841..298514	+	1674	WP_044487557.1	DUF4153 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24553.06 Da
• Isoelectric Point: 9.1953

>T21578 WP_011531703.1 NC_008027:292895-293536 [Pseudomonas entomophila L48]
MTYTRLNPSKALIWRVVHRDNLPWLLDNGLCAVSRGNLSSGYVGIGNPDLISKRSRRAVPIHPGGTLADYVPFYFTPFSV
MMKNIHSGRGVQQRRNEEIVILVSSLYHVRSLGLPFVFTDAHAYPDWAGYYSDLEHLDKIDWQILQSRDFKRDPDDPRKM
ERYQAEALIHGHVPVAGLLGIVCYNEALQRRVDQEVRQRQLDLPVHARPGWYF

Nucleotide

• Length: 642 bp

>T21578 NC_008027:292895-293536 [Pseudomonas entomophila L48]
ATGACGTATACCAGACTTAACCCAAGCAAAGCCCTGATCTGGCGAGTCGTCCACCGCGATAACCTCCCGTGGCTACTGGA
CAACGGCTTGTGTGCGGTTTCCAGAGGGAACCTCTCCAGCGGCTACGTCGGAATTGGTAATCCTGACTTGATCAGCAAGC
GTTCCAGGCGAGCGGTGCCCATCCATCCCGGTGGGACGCTGGCAGACTACGTGCCGTTCTACTTCACACCGTTCTCCGTG
ATGATGAAAAACATCCATTCAGGACGGGGAGTACAGCAACGTCGGAACGAGGAGATCGTGATTCTTGTCTCCAGCCTGTA
CCATGTGCGGTCCCTGGGGCTGCCCTTTGTCTTTACTGATGCTCATGCGTATCCGGACTGGGCAGGCTACTACAGCGACC
TGGAGCATCTGGACAAGATCGACTGGCAGATCTTGCAGTCCAGAGATTTCAAGCGTGACCCAGACGATCCACGAAAAATG
GAGCGCTATCAGGCTGAAGCCCTGATCCACGGGCACGTTCCAGTCGCTGGCCTTCTAGGGATTGTCTGCTACAACGAAGC
TTTGCAACGTCGTGTTGATCAGGAGGTCCGGCAACGTCAGCTGGATCTTCCCGTGCATGCTCGACCTGGGTGGTATTTCT
GA

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40495.36 Da
• Isoelectric Point: 5.6423

>AT21578 WP_011531704.1 NC_008027:293536-294624 [Pseudomonas entomophila L48]
MIRFTQGNLLEAKAEALVNTVNTVGVMGKGIALMFKERFVENYRLYAAACKADEVVTGKMFITEVRELDGPRWVVNFPTK
RHWRSPSQLAWITDGLHDLRSFILANDVSSIAIPPLGAGNGGLPWAAVREQIEVVLGDLDADVLVFEPTSKYQNVSKRTG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIERFTPDDNPLNLQFSAHKYGPYTNRLEHLLNNLDGSYLH
CDKRIGDAGPLDVIWFDEGRKAFVDTYLHSEAKVHLPALEFTAELIDGFESPFGMELLATVDWMLHRDGVAPTVDAIREG
LQNWSGGKEAAARKNALFDDDSLATALKKLSVFQAQGVVQVH

Nucleotide

• Length: 1089 bp

>AT21578 NC_008027:293536-294624 [Pseudomonas entomophila L48]
ATGATTCGCTTTACGCAAGGTAACCTGCTGGAGGCCAAGGCCGAAGCGCTGGTTAACACAGTCAACACCGTTGGGGTGAT
GGGTAAAGGCATTGCGCTGATGTTCAAAGAGCGCTTTGTAGAGAACTATCGCCTGTATGCGGCTGCCTGCAAAGCGGACG
AAGTTGTCACTGGAAAGATGTTCATCACCGAGGTTCGTGAGCTGGACGGCCCGCGCTGGGTTGTGAACTTTCCGACCAAG
CGTCATTGGCGTTCTCCATCACAGCTTGCCTGGATCACAGATGGCCTTCATGACTTGCGGAGCTTCATTCTTGCCAATGA
CGTGAGTTCCATAGCAATCCCTCCACTGGGGGCGGGTAATGGCGGGTTGCCTTGGGCTGCTGTGCGCGAACAGATTGAAG
TGGTGCTGGGGGATCTGGATGCTGACGTGCTCGTATTTGAGCCCACGAGCAAGTATCAAAATGTCTCGAAGCGCACAGGG
GTCGAGAAGCTTACCCCTGCCAGAGCGCTCATAGCCGAGCTGGTGCGTCGTTATTGGGTACTTGGTATGGAGTGCAGCTT
GCTGGAGATCCAGAAGCTGGCGTGGTTCCTGGAGCGCTCAATCGAGCGCTTCACTCCTGATGACAACCCGCTCAACCTGC
AGTTCAGTGCTCACAAGTACGGTCCTTACACTAACCGACTGGAGCATCTACTGAATAATCTCGATGGTAGTTATCTCCAT
TGTGACAAGCGTATCGGTGATGCTGGCCCCTTGGATGTGATCTGGTTCGATGAGGGGCGGAAGGCTTTCGTTGATACTTA
CTTGCACAGTGAGGCTAAGGTCCATTTACCTGCGTTGGAGTTTACGGCTGAGCTGATTGATGGCTTTGAGTCTCCATTTG
GTATGGAGCTTCTGGCCACGGTTGACTGGATGCTTCATCGGGATGGGGTGGCACCAACGGTCGATGCTATTCGGGAAGGG
TTGCAAAATTGGTCAGGTGGGAAGGAAGCAGCTGCCCGTAAGAATGCCCTCTTCGATGACGACTCTTTGGCAACTGCACT
AAAAAAGCTCTCGGTTTTCCAGGCTCAAGGCGTAGTGCAAGTACATTGA

Structures

Toxin

• AlphaFold DB: Q1IGF9

Antitoxin

• AlphaFold DB: Q1IGF8