Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5437395..5438020 Replicon chromosome
Accession NZ_CP082978
Organism Klebsiella pneumoniae strain BD-13

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K9F21_RS26900 Protein ID WP_002882817.1
Coordinates 5437395..5437778 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K9F21_RS26905 Protein ID WP_004150355.1
Coordinates 5437778..5438020 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F21_RS26885 (5434761) 5434761..5435663 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K9F21_RS26890 (5435660) 5435660..5436295 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K9F21_RS26895 (5436292) 5436292..5437221 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K9F21_RS26900 (5437395) 5437395..5437778 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F21_RS26905 (5437778) 5437778..5438020 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K9F21_RS26910 (5438225) 5438225..5439142 + 918 WP_032418237.1 alpha/beta hydrolase -
K9F21_RS26915 (5439156) 5439156..5440097 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K9F21_RS26920 (5440142) 5440142..5440579 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K9F21_RS26925 (5440576) 5440576..5441436 - 861 WP_004146232.1 virulence factor BrkB family protein -
K9F21_RS26930 (5441430) 5441430..5442029 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215772 WP_002882817.1 NZ_CP082978:c5437778-5437395 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215772 NZ_CP110947:740107-740592 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215772 WP_004150355.1 NZ_CP082978:c5438020-5437778 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215772 NZ_CP110947:739817-740110 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References