Detailed information of TA system TA215758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1899740..1900330
• Replicon: chromosome
• Accession: NZ_CP082978
• Organism: Klebsiella pneumoniae strain BD-13

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K9F21_RS09475
• Protein ID: WP_023341911.1
• Coordinates: 1899998..1900330 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: K9F21_RS09470
• Protein ID: WP_000288812.1
• Coordinates: 1899740..1899997 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F21_RS09445 (1895650)	1895650..1895724	+	75	Protein_1862	type II toxin-antitoxin system PemK/MazF family toxin	-
K9F21_RS09450 (1896075)	1896075..1897823	+	1749	WP_032418698.1	hypothetical protein	-
K9F21_RS09455 (1897877)	1897877..1898458	+	582	WP_099184326.1	hypothetical protein	-
K9F21_RS09460 (1898741)	1898741..1899202	+	462	WP_004213450.1	hypothetical protein	-
K9F21_RS09465 (1899199)	1899199..1899405	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
K9F21_RS09470 (1899740)	1899740..1899997	+	258	WP_000288812.1	antitoxin	Antitoxin
K9F21_RS09475 (1899998)	1899998..1900330	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9F21_RS09485 (1900652)	1900652..1902088	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
K9F21_RS09495 (1902454)	1902454..1903908	-	1455	WP_004148975.1	AMP nucleosidase	-
K9F21_RS09500 (1904038)	1904038..1904283	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T215758 WP_023341911.1 NZ_CP082978:1899998-1900330 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T215758 NZ_CP110941:510594-510878 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT215758 WP_000288812.1 NZ_CP082978:1899740-1899997 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT215758 NZ_CP110941:510362-510604 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0