Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 229919..230829 Replicon plasmid p1-BD-14-NDM1
Accession NZ_CP082976
Organism Klebsiella pneumoniae strain BD-14

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag K9F22_RS27175 Protein ID WP_004026354.1
Coordinates 229919..230389 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag K9F22_RS27180 Protein ID WP_004026357.1
Coordinates 230386..230829 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F22_RS27150 (K9F22_27085) 225893..226270 + 378 WP_000656305.1 GNAT family N-acetyltransferase -
K9F22_RS27155 (K9F22_27090) 226471..227130 - 660 WP_000412211.1 type A-1 chloramphenicol O-acetyltransferase -
K9F22_RS27160 (K9F22_27095) 227409..228106 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
K9F22_RS27165 (K9F22_27100) 228669..228827 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
K9F22_RS27170 (K9F22_27105) 229550..229810 + 261 WP_004026352.1 hypothetical protein -
K9F22_RS27175 (K9F22_27110) 229919..230389 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
K9F22_RS27180 (K9F22_27115) 230386..230829 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
K9F22_RS27185 (K9F22_27120) 230929..232200 - 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
K9F22_RS27190 (K9F22_27125) 232200..232622 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
K9F22_RS27195 (K9F22_27130) 232977..233546 - 570 Protein_255 transposase -
K9F22_RS27200 (K9F22_27135) 233548..233763 + 216 Protein_256 IS200/IS605 family transposase -
K9F22_RS27205 (K9F22_27140) 234648..235679 - 1032 WP_170919122.1 CRISPR-associated protein Csf2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / qnrS1 / aph(3')-VI / blaNDM-1 / catA1 - 1..342741 342741
- inside IScluster/Tn tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / qnrS1 / aph(3')-VI / blaNDM-1 / catA1 - 192717..237837 45120


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T215754 WP_004026354.1 NZ_CP082976:c230389-229919 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T215754 NZ_CP110936:c5263541-5263158 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT215754 WP_004026357.1 NZ_CP082976:c230829-230386 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT215754 NZ_CP110936:c5263783-5263541 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References