Detailed information of TA system TA215752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5232678..5233303
• Replicon: chromosome
• Accession: NZ_CP082975
• Organism: Klebsiella pneumoniae strain BD-14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: K9F22_RS25560
• Protein ID: WP_002882817.1
• Coordinates: 5232678..5233061 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K9F22_RS25565
• Protein ID: WP_162557435.1
• Coordinates: 5233061..5233303 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F22_RS25545 (5230044)	5230044..5230946	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
K9F22_RS25550 (5230943)	5230943..5231578	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
K9F22_RS25555 (5231575)	5231575..5232504	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
K9F22_RS25560 (5232678)	5232678..5233061	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9F22_RS25565 (5233061)	5233061..5233303	-	243	WP_162557435.1	CopG family transcriptional regulator	Antitoxin
K9F22_RS25570 (5233508)	5233508..5234425	+	918	WP_032417884.1	alpha/beta hydrolase	-
K9F22_RS25575 (5234440)	5234440..5235381	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
K9F22_RS25580 (5235426)	5235426..5235863	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
K9F22_RS25585 (5235860)	5235860..5236720	-	861	WP_004146232.1	virulence factor BrkB family protein	-
K9F22_RS25590 (5236714)	5236714..5237313	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T215752 WP_002882817.1 NZ_CP082975:c5233061-5232678 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T215752 NZ_CP110936:c4938302-4937955 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9557.79 Da
• Isoelectric Point: 4.3509

>AT215752 WP_162557435.1 NZ_CP082975:c5233303-5233061 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNISRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT215752 NZ_CP110936:c4937955-4937656 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8