Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4657715..4658525 Replicon chromosome
Accession NZ_CP082975
Organism Klebsiella pneumoniae strain BD-14

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K9F22_RS22820 Protein ID WP_004178461.1
Coordinates 4657715..4658248 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F22_RS22825 Protein ID WP_002887278.1
Coordinates 4658259..4658525 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F22_RS22815 (4656546) 4656546..4657667 + 1122 WP_004214138.1 cupin domain-containing protein -
K9F22_RS22820 (4657715) 4657715..4658248 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K9F22_RS22825 (4658259) 4658259..4658525 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F22_RS22830 (4658628) 4658628..4660061 - 1434 WP_032445081.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F22_RS22835 (4660051) 4660051..4660734 - 684 WP_032445080.1 copper response regulator transcription factor CusR -
K9F22_RS22840 (4660906) 4660906..4662291 + 1386 WP_032417819.1 efflux transporter outer membrane subunit -
K9F22_RS22845 (4662309) 4662309..4662653 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T215749 WP_004178461.1 NZ_CP082975:c4658248-4657715 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215749 NZ_CP110936:c3334701-3334599 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215749 WP_002887278.1 NZ_CP082975:c4658525-4658259 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215749 NZ_CP110936:3334592-3334737 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References