Detailed information of TA system TA215747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2611206..2612794
• Replicon: chromosome
• Accession: NZ_CP082975
• Organism: Klebsiella pneumoniae strain BD-14

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A6T928
• Locus tag: K9F22_RS12745
• Protein ID: WP_004176197.1
• Coordinates: 2611206..2612528 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K9F22_RS12750
• Protein ID: WP_032417111.1
• Coordinates: 2612528..2612794 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F22_RS12730 (2606262)	2606262..2608763	+	2502	WP_016831617.1	fimbria/pilus outer membrane usher protein	-
K9F22_RS12735 (2608751)	2608751..2609755	+	1005	WP_004176196.1	fimbrial protein	-
K9F22_RS12740 (2609897)	2609897..2611189	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
K9F22_RS12745 (2611206)	2611206..2612528	-	1323	WP_004176197.1	type II toxin-antitoxin system HipA family toxin	Toxin
K9F22_RS12750 (2612528)	2612528..2612794	-	267	WP_032417111.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
K9F22_RS12755 (2613059)	2613059..2613385	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
K9F22_RS12760 (2613382)	2613382..2613882	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
K9F22_RS12765 (2613860)	2613860..2615239	-	1380	WP_231538110.1	nucleobase:cation symporter-2 family protein	-
K9F22_RS12770 (2615585)	2615585..2616739	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
K9F22_RS12775 (2616720)	2616720..2617643	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2415471..2647647	232176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49111.41 Da
• Isoelectric Point: 7.5357

>T215747 WP_004176197.1 NZ_CP082975:c2612528-2611206 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T215747 NZ_CP110936:c1226616-1226398 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9926.32 Da
• Isoelectric Point: 8.5912

>AT215747 WP_032417111.1 NZ_CP082975:c2612794-2612528 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETASAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT215747 NZ_CP110936:c1227017-1226643 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A6T928