Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20872..21515 Replicon plasmid p3-BD-41
Accession NZ_CP082970
Organism Klebsiella pneumoniae strain BD-41

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K9F23_RS28475 Protein ID WP_001044770.1
Coordinates 21099..21515 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K9F23_RS28470 Protein ID WP_001261282.1
Coordinates 20872..21102 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F23_RS28435 (K9F23_28385) 16444..17310 - 867 WP_004118283.1 replication initiation protein -
K9F23_RS28440 (K9F23_28390) 17845..17949 + 105 WP_032409716.1 hypothetical protein -
K9F23_RS28445 (K9F23_28395) 18078..18335 + 258 WP_000764642.1 hypothetical protein -
K9F23_RS28450 (K9F23_28400) 18393..19169 - 777 WP_000015958.1 site-specific integrase -
K9F23_RS28455 (K9F23_28405) 19166..19909 - 744 WP_000129823.1 hypothetical protein -
K9F23_RS28460 (K9F23_28410) 19960..20310 - 351 WP_000493378.1 hypothetical protein -
K9F23_RS28465 (K9F23_28415) 20454..20915 - 462 WP_072202616.1 hypothetical protein -
K9F23_RS28470 (K9F23_28420) 20872..21102 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9F23_RS28475 (K9F23_28425) 21099..21515 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K9F23_RS28480 (K9F23_28430) 21589..22278 + 690 Protein_28 AAA family ATPase -
K9F23_RS28485 (K9F23_28435) 22333..23030 + 698 WP_223174630.1 IS1 family transposase -
K9F23_RS28490 (K9F23_28440) 23046..24203 - 1158 Protein_30 IS1380-like element ISEcp1 family transposase -
K9F23_RS28495 (K9F23_28445) 24397..24603 - 207 Protein_31 DUF4158 domain-containing protein -
K9F23_RS28500 (K9F23_28450) 24767..25324 + 558 WP_001217881.1 recombinase family protein -
K9F23_RS28505 (K9F23_28455) 25558..26112 + 555 WP_063840280.1 AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / dfrA14 - 1..48981 48981


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T215735 WP_001044770.1 NZ_CP082970:21099-21515 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215735 NZ_CP110934:c950717-950190 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCACTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT215735 WP_001261282.1 NZ_CP082970:20872-21102 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT215735 NZ_CP110934:c951004-950714 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References