Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53667..54403 Replicon plasmid p1-BD-41
Accession NZ_CP082968
Organism Klebsiella pneumoniae strain BD-41

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K9F23_RS27100 Protein ID WP_003026803.1
Coordinates 53921..54403 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K9F23_RS27095 Protein ID WP_003026799.1
Coordinates 53667..53933 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F23_RS27050 (K9F23_27015) 49729..50091 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
K9F23_RS27055 (K9F23_27020) 50141..50491 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
K9F23_RS27060 (K9F23_27025) 50849..51118 + 270 WP_004152102.1 hypothetical protein -
K9F23_RS27065 (K9F23_27030) 51106..51681 + 576 WP_004152103.1 hypothetical protein -
K9F23_RS27070 (K9F23_27035) 51712..52206 + 495 WP_004152104.1 DNA-binding protein -
K9F23_RS27075 (K9F23_27040) 52250..52618 + 369 WP_004152105.1 hypothetical protein -
K9F23_RS27080 (K9F23_27045) 52652..52855 + 204 WP_004152106.1 HHA domain-containing protein -
K9F23_RS27085 (K9F23_27050) 52904..53161 + 258 WP_004152107.1 hypothetical protein -
K9F23_RS27090 (K9F23_27055) 53237..53491 + 255 WP_004152108.1 hypothetical protein -
K9F23_RS27095 (K9F23_27060) 53667..53933 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K9F23_RS27100 (K9F23_27065) 53921..54403 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K9F23_RS27105 (K9F23_27070) 54604..56007 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
K9F23_RS27110 (K9F23_27075) 56036..56668 - 633 WP_001567369.1 hypothetical protein -
K9F23_RS27115 (K9F23_27080) 56898..58244 + 1347 WP_020314316.1 ISNCY family transposase -
K9F23_RS28895 58405..58536 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..212483 212483


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T215734 WP_003026803.1 NZ_CP082968:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T215734 NZ_CP110934:c815570-815157 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCTTTATGGATGATACTACTGTCACTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT215734 WP_003026799.1 NZ_CP082968:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT215734 NZ_CP110934:c815817-815551 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References