Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4781350..4782160 Replicon chromosome
Accession NZ_CP082967
Organism Klebsiella pneumoniae strain BD-41

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K9F23_RS23625 Protein ID WP_004178461.1
Coordinates 4781350..4781883 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K9F23_RS23630 Protein ID WP_002887278.1
Coordinates 4781894..4782160 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F23_RS23620 (4780181) 4780181..4781302 + 1122 WP_004214138.1 cupin domain-containing protein -
K9F23_RS23625 (4781350) 4781350..4781883 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K9F23_RS23630 (4781894) 4781894..4782160 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K9F23_RS23635 (4782263) 4782263..4783696 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
K9F23_RS23640 (4783686) 4783686..4784369 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K9F23_RS23645 (4784541) 4784541..4785926 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
K9F23_RS23650 (4785944) 4785944..4786288 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T215731 WP_004178461.1 NZ_CP082967:c4781883-4781350 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215731 NZ_CP110934:302013-302531 [Salmonella enterica]
GTGGACAACCTGACAATAGAGATTCTGGCTGATAACGCAGAATACAACTGGCGGCAGTTCGACTGCGGTGAGGCATCATT
AAACCTTTTTTTAACGCAGCATCTTCAGCGCCAGCATAACAACAAAATCTTGCGCGGTTATGTACTCAGAACCACTACGC
CAGAACGGCGAGTGTTGGGGTATTACACCCTGTCAGGAAGCTGCTTTGAACGCGCGTCGTTGCCTTCCAGAACCCAGCAA
AAGCGCATCCCTTACCAGAATATCCCCAGCGTTACGCTGGGCCGTTTAGCGGTCGATCTCTCATTACAGGGAAAAGGGTG
GGGAGCCATATTGGTCACGCATGCGATGAAAGTGGTGTGGTCGGCATCACTGGCCGTTGGGATTCATGGCATCTTTGTGG
AAGCGTTAAACGAAAAAGCCCAGGCCTTTTATCAGAGGCTGGGCTTTATTTCTTTATCCGGAGAAAACGAGCACGCGCTG
TTTTACCCGACAAAATCGATTGAGCAACTTTTTGGCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215731 WP_002887278.1 NZ_CP082967:c4782160-4781894 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215731 NZ_CP110934:301740-302009 [Salmonella enterica]
ATGTCAGCCCTAAAAAAACAGCGTATCGATCTGCGGCTCACAGACGGCGATAAAAGCATGATTGAAGAAGCGGCCGCCAT
TACTAATCAATCAATCACGCAATTTATGTTGAATAGCGCAGCGGAGCGAGCCGCAGAAGTGCTTGAACAGCACCGTCGGG
TTATTCTTAATGAAGCATCCTGGGCGCGAGTCATGGATGCGCTTAGTCATCCTCCCACCCCCAATGAGAAATTAAAACGC
GCGGCGCAACGTCTACAAGACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References