Detailed information of TA system TA021573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 5478690..5479282
• Replicon: chromosome
• Accession: NC_008009
• Organism: Candidatus Koribacter versatilis Ellin345

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ACID345_RS24045
• Protein ID: WP_148210247.1
• Coordinates: 5478935..5479282 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q1IHM7
• Locus tag: ACID345_RS24040
• Protein ID: WP_011525420.1
• Coordinates: 5478690..5478938 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACID345_RS24030	5474375..5475823	-	1449	WP_011525418.1	amidophosphoribosyltransferase	-
ACID345_RS24035	5476191..5478497	-	2307	WP_011525419.1	phosphoribosylformylglycinamidine synthase subunit PurL	-
ACID345_RS24040	5478690..5478938	+	249	WP_011525420.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ACID345_RS24045	5478935..5479282	+	348	WP_148210247.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACID345_RS24050	5479308..5480561	-	1254	WP_011525422.1	hypothetical protein	-
ACID345_RS24055	5480949..5481293	+	345	WP_011525423.1	PadR family transcriptional regulator	-
ACID345_RS24060	5481290..5484037	+	2748	WP_011525424.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-109)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12757.88 Da
• Isoelectric Point: 10.4496

>T21573 WP_148210247.1 NC_008009:5478935-5479282 [Candidatus Koribacter versatilis Ellin345]
VSTAYVPEAGDIVFLNFDPQVGREQAKRRPALVLTDLRYNRASGLAVVCPLTSKRKPYPFALPVTVDGVEGAVLVDQLKS
MDWTGRRATFHSKITPALFKKVRQYVAVLLSLREA

Nucleotide

• Length: 348 bp

>T21573 NC_008009:5478935-5479282 [Candidatus Koribacter versatilis Ellin345]
GTGAGCACCGCCTATGTTCCCGAGGCTGGTGACATTGTTTTCCTGAATTTCGATCCGCAAGTGGGGCGAGAACAAGCCAA
GCGTAGGCCAGCGCTGGTGCTGACGGATCTGCGGTACAACCGGGCGAGTGGATTGGCCGTGGTCTGCCCGCTGACGAGCA
AGAGGAAGCCATATCCTTTTGCGCTGCCTGTGACCGTCGATGGGGTAGAGGGTGCCGTGCTCGTCGATCAACTCAAGAGC
ATGGATTGGACGGGGCGACGCGCGACCTTCCACTCGAAGATCACGCCGGCGCTTTTCAAGAAGGTACGACAGTATGTCGC
GGTGTTGTTATCGCTGCGGGAAGCGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9005.24 Da
• Isoelectric Point: 4.7708

>AT21573 WP_011525420.1 NC_008009:5478690-5478938 [Candidatus Koribacter versatilis Ellin345]
MRAQMLKWGNSLAVRIPKAVAEQAKLAEGDALELEVASDGTVQLQRAGKTPTLSQLVAQITPENRYEETPWGADVGKEKV
EW

Nucleotide

• Length: 249 bp

>AT21573 NC_008009:5478690-5478938 [Candidatus Koribacter versatilis Ellin345]
ATGCGCGCGCAAATGTTGAAGTGGGGGAACAGTTTGGCGGTTCGGATTCCGAAGGCGGTTGCGGAGCAGGCGAAGTTGGC
GGAAGGCGATGCGTTGGAACTGGAAGTTGCTTCGGACGGGACAGTGCAGCTTCAGCGTGCGGGAAAGACTCCGACCTTGT
CGCAATTGGTAGCGCAGATCACGCCGGAGAACCGCTACGAAGAGACGCCGTGGGGCGCTGACGTGGGCAAGGAGAAAGTC
GAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: Q1IHM7