Detailed information of TA system TA215719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1842278..1842868
• Replicon: chromosome
• Accession: NZ_CP082967
• Organism: Klebsiella pneumoniae strain BD-41

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K9F23_RS09095
• Protein ID: WP_023341911.1
• Coordinates: 1842536..1842868 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: K9F23_RS09090
• Protein ID: WP_000288812.1
• Coordinates: 1842278..1842535 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F23_RS09065 (1838188)	1838188..1838262	+	75	Protein_1781	type II toxin-antitoxin system PemK/MazF family toxin	-
K9F23_RS09070 (1838613)	1838613..1840361	+	1749	WP_032418698.1	hypothetical protein	-
K9F23_RS09075 (1840415)	1840415..1840996	+	582	WP_099184326.1	hypothetical protein	-
K9F23_RS09080 (1841279)	1841279..1841740	+	462	WP_004213450.1	hypothetical protein	-
K9F23_RS09085 (1841737)	1841737..1841943	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
K9F23_RS09090 (1842278)	1842278..1842535	+	258	WP_000288812.1	antitoxin	Antitoxin
K9F23_RS09095 (1842536)	1842536..1842868	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K9F23_RS09105 (1843190)	1843190..1844626	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
K9F23_RS09115 (1844992)	1844992..1846446	-	1455	WP_004148975.1	AMP nucleosidase	-
K9F23_RS09120 (1846576)	1846576..1846821	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T215719 WP_023341911.1 NZ_CP082967:1842536-1842868 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T215719 NZ_CP110931:c804667-804269 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT215719 WP_000288812.1 NZ_CP082967:1842278-1842535 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT215719 NZ_CP110931:c804894-804667 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0