Detailed information of TA system TA215712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1734715..1735366
• Replicon: chromosome
• Accession: NZ_CP082961
• Organism: Lacticaseibacillus rhamnosus strain CAU 1365

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: K6087_RS07885
• Protein ID: WP_005686631.1
• Coordinates: 1734715..1735098 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: K6087_RS07890
• Protein ID: WP_005686632.1
• Coordinates: 1735118..1735366 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6087_RS07880 (K6087_07875)	1733674..1734201	-	528	WP_005691221.1	QueT transporter family protein	-
K6087_RS07885 (K6087_07880)	1734715..1735098	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K6087_RS07890 (K6087_07885)	1735118..1735366	-	249	WP_005686632.1	antitoxin	Antitoxin
K6087_RS07895 (K6087_07890)	1735478..1736617	-	1140	WP_005691215.1	alanine racemase	-
K6087_RS07900 (K6087_07895)	1736604..1736978	-	375	WP_005686634.1	holo-ACP synthase	-
K6087_RS07905 (K6087_07900)	1737147..1738655	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
K6087_RS07910 (K6087_07905)	1738929..1740317	-	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T215712 WP_005686631.1 NZ_CP082961:c1735098-1734715 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T215712 NZ_CP110921:c2252897-2252490 [Methylococcus sp. 16-5]
ATGAGTGTGCTGCACATGCTGGATACGGACATTGCGAGCTATGTGATCAAAGGCCGCTCTCCCGCCGTGGAAGCGAAACT
GATGGCGATCGTGCCGTCCATGGTTTGCATTTCCGCAGTGACCCGCGCTGAACTCATGTATGGGCTGAAACGTTTACTTG
CGGGGCACCGCCTCCACCTTGCGGTGCGCCGGTTTCTCAAGATCGTGCGGGTTCTGTCCTGGGACGCCGAGGCTGCAGAT
TATTACGCGGACATCCGCCACCAACTGGTTTCCACCGGCCAGCCCATTGGTGAGATGGACATGATGATCGCTGCCCATTC
ACTTTCCGCCGGCGCTATGCTGGTGACCAACAATGTCCGCCATTACAGCCGGATCGAGGCGCCTTTATTGTTAGTAAACT
GGGCTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT215712 WP_005686632.1 NZ_CP082961:c1735366-1735118 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT215712 NZ_CP110921:c2253166-2252894 [Methylococcus sp. 16-5]
ATGCCCGAAACGCGCATCGCCAAGCTGTTCAAGAACGGCGCCAGCCAGGCGGTTCGGCTGCCCGCCGAATTCCGCTTCGA
GGGGGATGAGGTTTACGCGACGCGGGATGAGGCGACCGGCGACGTGGTCTTGTCTTCCCGCCCCGGTGCGAAGACTTGGG
GCGACTTTTTCGAGCTGCTGCATTCCATCGAAGTCCCCGAGGATTTCATGGCTGAACGTCCCATGAACGTGGTACCCGCC
GAGCGCGACGTATTCGGGGGCGAAGAGCCATGA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19