Detailed information of TA system TA215711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1637807..1638369
• Replicon: chromosome
• Accession: NZ_CP082961
• Organism: Lacticaseibacillus rhamnosus strain CAU 1365

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C2JYM4
• Locus tag: K6087_RS07410
• Protein ID: WP_005692155.1
• Coordinates: 1637807..1638154 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K6087_RS07415
• Protein ID: WP_014571589.1
• Coordinates: 1638154..1638369 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6087_RS07390 (K6087_07385)	1633302..1634543	-	1242	WP_005692160.1	acyltransferase	-
K6087_RS07395 (K6087_07390)	1634540..1635241	-	702	WP_005692159.1	ATP-binding cassette domain-containing protein	-
K6087_RS07400 (K6087_07395)	1635234..1636052	-	819	WP_014571588.1	ABC transporter permease subunit	-
K6087_RS07405 (K6087_07400)	1636293..1636961	-	669	WP_005692157.1	phenylalanine--tRNA ligase beta subunit-related protein	-
K6087_RS07410 (K6087_07405)	1637807..1638154	-	348	WP_005692155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K6087_RS07415 (K6087_07410)	1638154..1638369	-	216	WP_014571589.1	hypothetical protein	Antitoxin
K6087_RS07420 (K6087_07415)	1638466..1640301	-	1836	WP_015764721.1	ABC transporter ATP-binding protein	-
K6087_RS07425 (K6087_07420)	1640288..1642078	-	1791	WP_015764722.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13150.01 Da
• Isoelectric Point: 7.9817

>T215711 WP_005692155.1 NZ_CP082961:c1638154-1637807 [Lacticaseibacillus rhamnosus]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Nucleotide

• Length: 348 bp

>T215711 NZ_CP110921:c2149236-2148949 [Methylococcus sp. 16-5]
ATGACCTATAGCCTCGAATTCCGGGACAGCGCCTGGAAGGAGTGGCAAAACCTCGACCGCCCCTTGCACGAGCAATTCAA
AGCCAAGCTGCTGGAGCGGCTGGCAAACCCCCGCGTCGAATCCGCCCGCCTTTCCGGTCTGCCGGACTGCTACAAGATCA
AGCTGCGCGCCGCCGGCTACCGGCTGGTGTATCAGGTGTTCGACGAGCGCGTGGTGGTCGTGGTCGTGGCCGTGGGCAAG
CGGGAAGACAGTGCGGTGTATCGCAAGGCCAGGGGACGGGTGAAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7986.13 Da
• Isoelectric Point: 5.7995

>AT215711 WP_014571589.1 NZ_CP082961:c1638369-1638154 [Lacticaseibacillus rhamnosus]
MDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Nucleotide

• Length: 216 bp

>AT215711 NZ_CP110921:c2149471-2149226 [Methylococcus sp. 16-5]
ATGGAACTGCTCTACACCAATGCCTCGGTCAGCATCAGCGAACTGAAGAAGAACCCCACCGCGGTCATCGAAGAGGCAGA
CGGCTTCCCCGTCGCCGTGCTCAACCACAACAAGCCTGCTGCCTACCTGGTGCCCGCTGCCGCCTTCGAAGCCATGATGG
AAAAACTCGACGACATCGAACTCGCCAGGCTGGTGCGGGAGCGCGAGAACGAACCCACTGTCCGGGTCGATCCCCATGAC
CTATAG

Structures

Toxin

• AlphaFold DB: A0A7Z9DS75