Detailed information of TA system TA215701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4735493..4736109
• Replicon: chromosome
• Accession: NZ_CP082954
• Organism: Salmonella enterica subsp. arizonae strain S499

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E9YCX3
• Locus tag: K8I78_RS22655
• Protein ID: WP_023249114.1
• Coordinates: 4735493..4735867 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: K8I78_RS22660
• Protein ID: WP_001523745.1
• Coordinates: 4735867..4736109 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8I78_RS22640 (4732995)	4732995..4733897	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
K8I78_RS22645 (4733894)	4733894..4734529	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
K8I78_RS22650 (4734526)	4734526..4735455	+	930	WP_053509958.1	formate dehydrogenase accessory protein FdhE	-
K8I78_RS22655 (4735493)	4735493..4735867	-	375	WP_023249114.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8I78_RS22660 (4735867)	4735867..4736109	-	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
K8I78_RS22665 (4736314)	4736314..4737240	+	927	Protein_4427	alpha/beta hydrolase	-
K8I78_RS22670 (4737326)	4737326..4737637	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
K8I78_RS22675 (4737634)	4737634..4738080	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
K8I78_RS22680 (4738095)	4738095..4739036	-	942	WP_024134888.1	fatty acid biosynthesis protein FabY	-
K8I78_RS22685 (4739081)	4739081..4739518	-	438	WP_023249111.1	D-aminoacyl-tRNA deacylase	-
K8I78_RS22690 (4739515)	4739515..4740387	-	873	WP_088760336.1	virulence factor BrkB family protein	-
K8I78_RS22695 (4740381)	4740381..4740980	-	600	WP_000965699.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13836.12 Da
• Isoelectric Point: 8.0689

>T215701 WP_023249114.1 NZ_CP082954:c4735867-4735493 [Salmonella enterica subsp. arizonae]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNVIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T215701 NZ_CP110894:c4787948-4787589 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACTTGCTTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTTTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCAGGGGTTC
TGGAGGTCGTTGAGGATTACCACGGTAGCGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAATCCGTTTCCGGGAAGGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCATATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT215701 WP_001523745.1 NZ_CP082954:c4736109-4735867 [Salmonella enterica subsp. arizonae]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT215701 NZ_CP110894:c4787599-4787282 [Citrobacter freundii]
ATGTCATATGAAGTGGAAGCCAGTAGCGGTAATGTTTACGTCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGTTACGCGGTCAGTTTCGAGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGACTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA