Detailed information of TA system TA215688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 3722..4271
• Replicon: plasmid pCl300
• Accession: NZ_CP082953
• Organism: Acinetobacter baumannii strain Cl300

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9KJ78
• Locus tag: K9C16_RS19270
• Protein ID: WP_000176412.1
• Coordinates: 3722..4012 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9KCM7
• Locus tag: K9C16_RS19275
• Protein ID: WP_005140357.1
• Coordinates: 3996..4271 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9C16_RS19245 (K9C16_19160)	135..1016	+	882	WP_005804946.1	replication initiation protein RepM	-
K9C16_RS19250 (K9C16_19165)	1080..1646	+	567	WP_000858429.1	plasmid replication DNA-binding protein	-
K9C16_RS19255 (K9C16_19170)	1898..2227	-	330	WP_000610966.1	four-helix bundle copper-binding protein	-
K9C16_RS19260 (K9C16_19175)	3010..3531	+	522	WP_000999915.1	hypothetical protein	-
K9C16_RS19265 (K9C16_19180)	3528..3674	+	147	WP_000192351.1	hypothetical protein	-
K9C16_RS19270 (K9C16_19185)	3722..4012	-	291	WP_000176412.1	type II toxin-antitoxin system YafQ family toxin	Toxin
K9C16_RS19275 (K9C16_19190)	3996..4271	-	276	WP_005140357.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K9C16_RS19280 (K9C16_19195)	4475..4699	-	225	WP_002072934.1	hypothetical protein	-
K9C16_RS19285 (K9C16_19200)	4848..5090	+	243	WP_001015364.1	hypothetical protein	-
K9C16_RS19290 (K9C16_19205)	5163..6308	-	1146	WP_000027028.1	hypothetical protein	-
K9C16_RS19295 (K9C16_19210)	6587..7363	-	777	WP_000191151.1	hypothetical protein	-
K9C16_RS19300 (K9C16_19215)	7632..8792	+	1161	WP_000179382.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9205	9205

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (9-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11187.12 Da
• Isoelectric Point: 6.9783

>T215688 WP_000176412.1 NZ_CP082953:c4012-3722 [Acinetobacter baumannii]
MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTVPDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDN
NELILARIGSHSELFG

Nucleotide

• Length: 291 bp

>T215688 NZ_CP110892:c157637-157227 [Citrobacter freundii]
GTGAACAGGACATATATGCTCGACACCCGTCTCTGCGCGTACATCATGCGCGAGCAGCCGGAAGCGGTACTGACACGCCT
GGAGCAGGCGGTGCTGCGCGGCGACCGTATCGTGGTCTCTGCCGTCACCTGGGCCGAACTGTCACAGGCGGCACGGGCGT
CCGGTCCCGCCACGCAGGCACTGGCCGACGCGTTCTGCGCCAGTCTCGATGCCGTGCTGGCCTGGGATCGCGCCGCGGTG
GATGCCACCACCGTCATTAAGGCAGCACTGGCCGCAGACGGCACGCCGATCGGCCCGAATGACACGGCGATCGCTGGGCA
TGCCATCGCCGCCGGCGCCGTGCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGGAAGACT
GGGTTAAATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10290.65 Da
• Isoelectric Point: 4.6702

>AT215688 WP_005140357.1 NZ_CP082953:c4271-3996 [Acinetobacter baumannii]
MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQVVNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQE
LFTDLEDDTDD

Nucleotide

• Length: 276 bp

>AT215688 NZ_CP110892:c157864-157634 [Citrobacter freundii]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGGGAAACTGTCTTCAGTGACCAGGGCCGGGGTGAGCAGTGA

Structures

Toxin

• AlphaFold DB: N9KJ78

Antitoxin

• AlphaFold DB: N9KCM7