215685

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2606002..2606590 Replicon chromosome
Accession NZ_CP082952
Organism Acinetobacter baumannii strain Cl300

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag K9C16_RS12500 Protein ID WP_000438825.1
Coordinates 2606002..2606289 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag K9C16_RS12505 Protein ID WP_004728120.1
Coordinates 2606276..2606590 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9C16_RS12465 (K9C16_12415) 2601051..2601398 - 348 Protein_2444 IS4 family transposase -
K9C16_RS12470 (K9C16_12420) 2601488..2602303 + 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
K9C16_RS12475 (K9C16_12425) 2602390..2602692 + 303 WP_000251875.1 phosphoglucosamine mutase -
K9C16_RS12480 (K9C16_12430) 2602868..2603698 - 831 Protein_2447 IS91-like element ISVsa3 family transposase -
K9C16_RS12485 (K9C16_12435) 2603762..2604466 + 705 WP_071543226.1 IS6-like element IS1006 family transposase -
K9C16_RS12490 (K9C16_12440) 2604594..2604932 - 339 WP_032073153.1 hypothetical protein -
K9C16_RS12495 (K9C16_12445) 2604991..2605761 + 771 WP_032073134.1 hypothetical protein -
K9C16_RS12500 (K9C16_12450) 2606002..2606289 + 288 WP_000438825.1 BrnT family toxin Toxin
K9C16_RS12505 (K9C16_12455) 2606276..2606590 + 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
K9C16_RS12510 (K9C16_12460) 2606679..2607209 - 531 WP_000172759.1 hypothetical protein -
K9C16_RS12515 (K9C16_12465) 2607358..2607660 + 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
K9C16_RS12520 (K9C16_12470) 2607661..2607942 + 282 WP_000985609.1 putative addiction module antidote protein -
K9C16_RS12525 (K9C16_12475) 2608022..2608441 - 420 WP_001180106.1 SMI1/KNR4 family protein -
K9C16_RS12530 (K9C16_12480) 2608604..2609938 + 1335 WP_033917544.1 ISNCY family transposase -
K9C16_RS12535 (K9C16_12485) 2610067..2610387 + 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
K9C16_RS12540 (K9C16_12490) 2610380..2610652 + 273 WP_000369781.1 NadS family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage sul2 - 2597129..2610387 13258
- inside IScluster/Tn sul2 / msr(E) - 2600268..2613221 12953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T215685 WP_000438825.1 NZ_CP082952:2606002-2606289 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T215685 NZ_CP110891:190030-190338 [Citrobacter freundii]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT215685 WP_004728120.1 NZ_CP082952:2606276-2606590 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT215685 NZ_CP110891:189788-190027 [Citrobacter freundii]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References