Detailed information of TA system TA021568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2558158..2559200
• Replicon: chromosome
• Accession: NC_007973
• Organism: Cupriavidus metallidurans CH34

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: RMET_RS11760
• Protein ID: WP_003153636.1
• Coordinates: 2558158..2558733 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: RMET_RS11765
• Protein ID: WP_003050245.1
• Coordinates: 2558730..2559200 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RMET_RS11735	2554596..2555321	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
RMET_RS11740	2555360..2556262	-	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
RMET_RS11745	2556262..2556762	-	501	WP_003090159.1	hypothetical protein	-
RMET_RS11750	2556759..2557229	-	471	WP_003153638.1	hypothetical protein	-
RMET_RS11755	2557226..2558140	-	915	WP_003050256.1	AAA family ATPase	-
RMET_RS11760	2558158..2558733	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
RMET_RS11765	2558730..2559200	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
RMET_RS11770	2559404..2559787	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
RMET_RS11775	2559784..2560017	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
RMET_RS11780	2560034..2560393	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
RMET_RS11785	2560406..2560804	+	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
RMET_RS11790	2560801..2561493	+	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
RMET_RS11795	2561490..2562401	+	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
RMET_RS11800	2562391..2563809	+	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2524369..2599412	75043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T21568 WP_003153636.1 NC_007973:c2558733-2558158 [Cupriavidus metallidurans CH34]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T21568 NC_007973:c2558733-2558158 [Cupriavidus metallidurans CH34]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT21568 WP_003050245.1 NC_007973:c2559200-2558730 [Cupriavidus metallidurans CH34]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT21568 NC_007973:c2559200-2558730 [Cupriavidus metallidurans CH34]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68