Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 245660..246296 Replicon chromosome
Accession NZ_CP082944
Organism Metabacillus dongyingensis strain BY2G20

Toxin (Protein)


Gene name mazF Uniprot ID A0A2N5FBL1
Locus tag K8L98_RS01365 Protein ID WP_029286376.1
Coordinates 245946..246296 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2N5FBR6
Locus tag K8L98_RS01360 Protein ID WP_029286378.1
Coordinates 245660..245941 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8L98_RS01340 (K8L98_01340) 241690..242283 - 594 WP_223439017.1 rhomboid family intramembrane serine protease -
K8L98_RS01345 (K8L98_01345) 242386..242751 + 366 WP_223439018.1 holo-ACP synthase -
K8L98_RS01350 (K8L98_01350) 242897..243907 + 1011 WP_223439019.1 outer membrane lipoprotein carrier protein LolA -
K8L98_RS01355 (K8L98_01355) 244332..245513 + 1182 WP_223439020.1 alanine racemase -
K8L98_RS01360 (K8L98_01360) 245660..245941 + 282 WP_029286378.1 YlcI/YnfO family protein Antitoxin
K8L98_RS01365 (K8L98_01365) 245946..246296 + 351 WP_029286376.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K8L98_RS01370 (K8L98_01370) 246434..247270 + 837 WP_223439021.1 STAS domain-containing protein -
K8L98_RS01375 (K8L98_01375) 247267..247632 + 366 WP_101569209.1 STAS domain-containing protein -
K8L98_RS01380 (K8L98_01380) 247635..248036 + 402 WP_070878720.1 anti-sigma regulatory factor -
K8L98_RS01385 (K8L98_01385) 248048..249058 + 1011 WP_223439022.1 PP2C family protein-serine/threonine phosphatase -
K8L98_RS01390 (K8L98_01390) 249121..249453 + 333 WP_223439023.1 anti-sigma factor antagonist -
K8L98_RS01395 (K8L98_01395) 249450..249932 + 483 WP_223439024.1 anti-sigma B factor RsbW -
K8L98_RS01400 (K8L98_01400) 249898..250686 + 789 WP_070878724.1 RNA polymerase sigma factor SigB -
K8L98_RS01405 (K8L98_01405) 250686..251285 + 600 WP_223439025.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13036.06 Da        Isoelectric Point: 4.8781

>T215676 WP_029286376.1 NZ_CP082944:245946-246296 [Metabacillus dongyingensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDSKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T215676 NZ_CP110887:c3382924-3382721 [Dickeya solani]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCTGCAGATCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAGATACGTTCGCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10572.01 Da        Isoelectric Point: 7.0156

>AT215676 WP_029286378.1 NZ_CP082944:245660-245941 [Metabacillus dongyingensis]
VSESSATTEILIRLPQALVSELDGLVKQENGNRSELIYQATKMYIRERKKRQIRESMRRGYMEMAKINLNIASEAFQAES
EADHTVERLVSGG

Download         Length: 282 bp

>AT215676 NZ_CP110887:c3383346-3382978 [Dickeya solani]
ATGGATGAGTACACACCCCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCATGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGCTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATCAATGACACCGAGTTGCGGAAATGGCAAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N5FBL1

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5FBR6

References