215674

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 171529..172439 Replicon plasmid unnamed2
Accession NZ_CP082938
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag K8M01_RS27325 Protein ID WP_004181896.1
Coordinates 171969..172439 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag K8M01_RS27320 Protein ID WP_004181895.1
Coordinates 171529..171972 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS27295 (K8M01_27220) 166685..166900 - 216 Protein_182 IS200/IS605 family transposase -
K8M01_RS27300 (K8M01_27225) 166902..167471 + 570 Protein_183 transposase -
K8M01_RS27305 (K8M01_27230) 168162..169649 + 1488 WP_160458682.1 group II intron reverse transcriptase/maturase -
K8M01_RS27310 (K8M01_27235) 169735..170157 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
K8M01_RS27315 (K8M01_27240) 170157..171428 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
K8M01_RS27320 (K8M01_27245) 171529..171972 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
K8M01_RS27325 (K8M01_27250) 171969..172439 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
K8M01_RS27330 (K8M01_27255) 172549..172809 - 261 WP_004026352.1 hypothetical protein -
K8M01_RS27335 (K8M01_27260) 173499..174868 + 1370 WP_085956500.1 IS3-like element ISKpn8 family transposase -
K8M01_RS27340 (K8M01_27265) 174951..175997 - 1047 WP_004173931.1 IS481-like element ISKpn28 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..299755 299755
- flank IS/Tn - - 167085..167471 386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T215674 WP_004181896.1 NZ_CP082938:171969-172439 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T215674 NZ_CP110887:c2758745-2758440 [Dickeya solani]
ATGCAATTCATTGTTTATGAATACAAACGAGCCAGTCACTACAAAATGTTTGTCGATGTGCAGAGTGATATCGTCGAGAC
ACCCAAGCGGCGCATGGTCATTCCGCTTATCGAAGCACACCACCTGTCTGAGAAAGTGAATAAGACGTTGTTCCCTAAGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTACCTGTTGAAGTCATGGGTGAAGTGATT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT215674 WP_004181895.1 NZ_CP082938:171529-171972 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT215674 NZ_CP110887:c2758966-2758748 [Dickeya solani]
ATGAAACACCGCGTCAGTGTTACCGTAGACAAAGACAACTATCAGGTTCTGAGTGCTGCCGGAGTCAACATTTCTGGGCT
GGTGAACGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGGAGTGGAAGAAAGAAAACAGTGAGGGGATGGAGG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References