Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 68990..69515 Replicon plasmid unnamed1
Accession NZ_CP082937
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag K8M01_RS26065 Protein ID WP_013023785.1
Coordinates 68990..69295 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag K8M01_RS26070 Protein ID WP_001568025.1
Coordinates 69297..69515 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS26035 (K8M01_25990) 64677..65303 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
K8M01_RS26040 65300..65602 + 303 WP_004197636.1 hypothetical protein -
K8M01_RS26045 (K8M01_26000) 66066..66860 - 795 WP_004197635.1 site-specific integrase -
K8M01_RS26050 (K8M01_26005) 67058..68074 - 1017 WP_017899884.1 hypothetical protein -
K8M01_RS26055 (K8M01_26010) 68085..68399 - 315 WP_053389906.1 hypothetical protein -
K8M01_RS26060 (K8M01_26015) 68426..68821 - 396 WP_017899885.1 hypothetical protein -
K8M01_RS26065 (K8M01_26020) 68990..69295 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
K8M01_RS26070 (K8M01_26025) 69297..69515 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K8M01_RS26075 (K8M01_26030) 70333..70623 - 291 WP_013023783.1 hypothetical protein -
K8M01_RS26080 (K8M01_26035) 70620..71747 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
K8M01_RS26085 (K8M01_26040) 71781..73304 - 1524 WP_017899887.1 hypothetical protein -
K8M01_RS26090 (K8M01_26045) 73580..74359 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..122148 122148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T215672 WP_013023785.1 NZ_CP082937:c69295-68990 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T215672 NZ_CP110887:2154343-2155662 [Dickeya solani]
ATGGCGGCGCTCGATGTTTACATGAACGGCTACCGGGTGGGAATTCTGACCAAAACGGGCAGTGGCGCGCATCATTTTTC
CTATGATGCGAACTGGCTCGAGTTGCCGGGGAGTCGCCCCCTCTCGCTCTCAATGCCGCTACGCCATCAGCCCTATCAAG
GTGATGAAGTTTATAATTTCTTCGACAACTTGTTGCCGGATAACCCGGATATCAGGCGGCGGATTGTCACCAGGCATCAG
GCGGATTCCACCCAGCCGTTTGATTTGCTGGCTAAAGTGGGGCAAGACAGTGTTGGGGCGTTGCAATTGGTGCCGCAGGG
CACCGCTGTTGGCGATATAAAACAGATCGAATACAAAACCTTATCCGAGCAACAGCTTGAAAACATACTGGCGGGATATC
TGTCCGATGCCCCGCTCGGGATGATTGATACAGAAGAGGATTTTCGTATCTCTATCGCAGGCGCACAGGAAAAGACGGCG
CTGCTTTATCTGGATGGCAGCTGGTGTTTGCCTCTTAATACCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATTGAGAGTCATTCATACTCAATTGATATGTCTGACAGCGTTGAAAATGAATACCTGTGCCTGCTCATTGCCAAGGCGT
TTGGGCTGTCGGTACCGCACTGTTTTATGATCACAGCAGGCAAGATAAAAGCGCTGGCGGTAGAACGTTTTGATCGTAAA
TACGCCTCGGATGGTCGTTGGATTATGCGGTTGCCGCAGGAGGATTTTTGCCAGGTATTAAACGTGCCTTCCGCACGAAA
ATACGAAAATCATGGTGGACCGGGCATTTCCGCTATTATGTCGGTGCTATTAGGGGCGATTGAACCTGAAAGGGATCGCT
ATACCTTTATGAAGGCGCAGGTGCTGTTTTGGCTATTGGCTGCAACGGATGGACATGCAAAAAACTTCTCCCTGTTTATT
GAATTTGAAGGACGATATCGCCTCACGCCTTTCTATGACATTCTCTCTATGTACCCGGCGATGGGCGGGCGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGGCTGACCGGCTCAAAAGGCAAGAAATACGCCATTGAGCAGATCTTTCCACGGC
ACTTTTTCCAAACGGCAAAGGTGGTTGGATTTGCCAGAGAATCGATGGAACGCATTCTGACTGAATTTTCACAGACGGTT
GATTCTGTCATCGTGAGTGTCAGAAACCAACTGCCTGCGGATTTTCCCACTCCTATCCGCGATGCGATCCTGGGAGGGCT
ACAGACTAGATCGAGTCGATTGACGACGGGCTGGGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT215672 WP_001568025.1 NZ_CP082937:c69515-69297 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT215672 NZ_CP110887:2154074-2154343 [Dickeya solani]
ATGAAGGTCACCAACAGCAAACAGCTTAGCAGCTATCTAAAAGATGTTCGTATCACGCAGAAGCTTTCCCAAGGGAAAGT
GGCCAGTAAAGTGGGGATTCGTCAGGACACGGTATCGAGCTTCGAACAACATCCCGATTCCACCAAGCTGGAAACCTTCT
TTAAGATCCTGTCGGCATTAAATTTAGAACTTACCATTACCCCCAGAAATGCGCATGCAGCCAATAACGAGGCTTCTGTT
GCAACATCAGCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References