Detailed information of TA system TA021567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1169790..1170403
• Replicon: chromosome
• Accession: NC_007973
• Organism: Cupriavidus metallidurans CH34

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q1LPG3
• Locus tag: RMET_RS05415
• Protein ID: WP_011515865.1
• Coordinates: 1170224..1170403 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RMET_RS05410
• Protein ID: WP_029310017.1
• Coordinates: 1169790..1170194 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RMET_RS05395	1165180..1165713	+	534	WP_029308195.1	peptidylprolyl isomerase	-
RMET_RS05400	1165808..1166860	-	1053	WP_011515862.1	hypothetical protein	-
RMET_RS05405	1166857..1169463	-	2607	WP_035821829.1	DNA mismatch repair protein MutS	-
RMET_RS05410	1169790..1170194	-	405	WP_029310017.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RMET_RS05415	1170224..1170403	-	180	WP_011515865.1	type II toxin-antitoxin system HicA family toxin	Toxin
RMET_RS05420	1170607..1171428	-	822	WP_011515866.1	inositol monophosphatase	-
RMET_RS05425	1171656..1172525	+	870	WP_011515867.1	RNA methyltransferase	-
RMET_RS05430	1172854..1173597	+	744	WP_011515868.1	serine O-acetyltransferase	-
RMET_RS05435	1173646..1174437	-	792	WP_011515869.1	UDP-2,3-diacylglucosamine diphosphatase	-
RMET_RS05440	1174506..1175006	-	501	WP_011515870.1	peptidyl-prolyl cis-trans isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6790.07 Da
• Isoelectric Point: 12.3707

>T21567 WP_011515865.1 NC_007973:c1170403-1170224 [Cupriavidus metallidurans CH34]
MHSSTLIRLLEQKGWKLVRTHGSHFTFKHPRHMLIVTVPHPKKDLPTGTVRNILRTAGL

Nucleotide

• Length: 180 bp

>T21567 NC_007973:c1170403-1170224 [Cupriavidus metallidurans CH34]
TTGCATTCGTCCACGCTGATCCGATTGCTCGAACAGAAGGGGTGGAAGCTGGTGCGCACGCATGGCAGTCATTTCACCTT
CAAACATCCGCGGCACATGCTGATTGTGACCGTGCCCCATCCGAAGAAGGATCTGCCGACAGGCACGGTCCGAAACATTC
TGAGGACGGCCGGACTATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14618.79 Da
• Isoelectric Point: 4.9285

>AT21567 WP_029310017.1 NC_007973:c1170194-1169790 [Cupriavidus metallidurans CH34]
MDFPIAIHKDANSVYGVTVPDVAGCYSAGESIEEAVRNAREAITTHVVALLSLGEEVVLKTTPIGTLMAQPEYADAIWAL
ADIDMSKLDARPERINISLPRFVLHKIDMFVERRHETRSGFLARVALDAIAGSV

Nucleotide

• Length: 405 bp

>AT21567 NC_007973:c1170194-1169790 [Cupriavidus metallidurans CH34]
ATGGACTTTCCCATTGCCATTCACAAGGATGCGAACTCGGTCTACGGCGTAACGGTGCCCGATGTGGCGGGCTGTTACTC
CGCCGGCGAATCGATCGAGGAAGCGGTCCGCAACGCACGCGAGGCCATCACGACTCACGTGGTGGCCCTGCTCTCCCTTG
GCGAGGAGGTTGTTTTGAAAACGACGCCGATCGGCACGCTGATGGCCCAACCTGAGTACGCAGACGCGATCTGGGCGCTG
GCCGACATCGACATGAGCAAGCTCGACGCACGCCCGGAGCGAATCAATATCAGCCTGCCTCGTTTCGTATTGCACAAGAT
CGACATGTTCGTGGAACGCCGCCACGAAACCCGGAGCGGGTTCCTCGCGCGCGTCGCGCTGGACGCCATCGCGGGCAGCG
TCTGA

Structures

Toxin

• AlphaFold DB: A0A2L0WZI7