Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3914766..3915391 Replicon chromosome
Accession NZ_CP082936
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K8M01_RS18990 Protein ID WP_002882817.1
Coordinates 3914766..3915149 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K8M01_RS18995 Protein ID WP_004150355.1
Coordinates 3915149..3915391 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS18975 (3912132) 3912132..3913034 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K8M01_RS18980 (3913031) 3913031..3913666 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K8M01_RS18985 (3913663) 3913663..3914592 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K8M01_RS18990 (3914766) 3914766..3915149 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K8M01_RS18995 (3915149) 3915149..3915391 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K8M01_RS19000 (3915596) 3915596..3916513 + 918 WP_004178029.1 alpha/beta hydrolase -
K8M01_RS19005 (3916527) 3916527..3917468 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K8M01_RS19010 (3917513) 3917513..3917950 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K8M01_RS19015 (3917947) 3917947..3918807 - 861 WP_004146232.1 virulence factor BrkB family protein -
K8M01_RS19020 (3918801) 3918801..3919400 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215668 WP_002882817.1 NZ_CP082936:c3915149-3914766 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215668 NZ_CP110886:4042822-4043136 [Dickeya solani]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCTGTTTACCCGCTGTTGCTCGATGTCACAAGCGATATTATTGG
GCAGTTGAATCGCCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTATCCGGCAGGCCGCCGACCAGATCGTCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCACTGGGG
GCGGTGTTTTGTGATGCGTCTCAGTATCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATCGACGGCATTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215668 WP_004150355.1 NZ_CP082936:c3915391-3915149 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215668 NZ_CP110886:4042583-4042819 [Dickeya solani]
ATGACCGCGAAACAACGCAACACGCAAAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
CATTAACCTGAGTGCCATCCTGACAACCGCCCTGGATGCAGAGCTTCGCCACCATGAAGCAAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAACCGTTTTCATGATGAAAATGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References