215666

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3336765..3337575 Replicon chromosome
Accession NZ_CP082936
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K8M01_RS16250 Protein ID WP_002887280.1
Coordinates 3336765..3337298 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K8M01_RS16255 Protein ID WP_002887278.1
Coordinates 3337309..3337575 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS16245 (3335596) 3335596..3336717 + 1122 WP_004177557.1 cupin domain-containing protein -
K8M01_RS16250 (3336765) 3336765..3337298 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K8M01_RS16255 (3337309) 3337309..3337575 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K8M01_RS16260 (3337678) 3337678..3339111 - 1434 WP_004177561.1 Cu(+)/Ag(+) sensor histidine kinase -
K8M01_RS16265 (3339101) 3339101..3339784 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K8M01_RS16270 (3339956) 3339956..3341341 + 1386 WP_004177562.1 efflux transporter outer membrane subunit -
K8M01_RS16275 (3341359) 3341359..3341703 + 345 WP_025983237.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T215666 WP_002887280.1 NZ_CP082936:c3337298-3336765 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T215666 NZ_CP110886:3844469-3844726 [Dickeya solani]
ATGCCTGAGGTTGATAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTATGATGATGAGCGGCACTACTTGCCGCATGT
ACATGTCTGCTACGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACCGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGATGGCGCTTGCGCATATCAGGGCCCATCGGGAACAACTGCTGAGTATGTGGGAACGGGCGGTAGTGGGGAGA
AATCCAGGAAAATTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT215666 WP_002887278.1 NZ_CP082936:c3337575-3337309 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT215666 NZ_CP110886:3844734-3845249 [Dickeya solani]
ATGCATAAAATCGTGGAAGTTGACGTTATTGGTGATTTTTCGTTGCGCCTGACCTATGACGATGGTCTGGTTTGCGACAT
TAATCTTTATCAATATGCGTCCAGCCCAGTATTTTCGGACGTCAGCCTATTTATCAAGTTTGGGTTGTTGCCGGATGGCG
CGTTGGAATGGCTGCCGGATATTAAGATTTCCGCGTCAGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTGGCG
GTGCGAGAAAGGCACTTCGCCGATGTCATCTCGCGCGCCTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCAGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACCGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGCT
TGTATAAGTCGCTGAATAAAAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAGTTGGAA
GAGTTGCAATTGGAAGATCTGCAATTGGAAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLZ1

References