Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2752113..2752732 Replicon chromosome
Accession NZ_CP082936
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K8M01_RS13420 Protein ID WP_002892050.1
Coordinates 2752514..2752732 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K8M01_RS13415 Protein ID WP_002892066.1
Coordinates 2752113..2752487 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS13405 (2747265) 2747265..2748458 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K8M01_RS13410 (2748481) 2748481..2751627 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K8M01_RS13415 (2752113) 2752113..2752487 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K8M01_RS13420 (2752514) 2752514..2752732 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K8M01_RS13425 (2752891) 2752891..2753457 + 567 WP_002892030.1 maltose O-acetyltransferase -
K8M01_RS13430 (2753429) 2753429..2753569 - 141 WP_004147370.1 hypothetical protein -
K8M01_RS13435 (2753590) 2753590..2754060 + 471 WP_002892026.1 YlaC family protein -
K8M01_RS13440 (2754035) 2754035..2755486 - 1452 WP_004177237.1 PLP-dependent aminotransferase family protein -
K8M01_RS13445 (2755587) 2755587..2756285 + 699 WP_004177238.1 GNAT family protein -
K8M01_RS13450 (2756282) 2756282..2756422 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K8M01_RS13455 (2756422) 2756422..2756685 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215665 WP_002892050.1 NZ_CP082936:2752514-2752732 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215665 NZ_CP110886:c3382979-3382776 [Dickeya solani]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCTGCAGATCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAGATACGTTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215665 WP_002892066.1 NZ_CP082936:2752113-2752487 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215665 NZ_CP110886:c3383401-3383033 [Dickeya solani]
ATGGATGAGTACACACCCCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCATGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGCTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATCAATGACACCGAGTTGCGGAAATGGCAAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References