Detailed information of TA system TA021566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 29996..30561
• Replicon: plasmid pMOL30
• Accession: NC_007971
• Organism: Cupriavidus metallidurans CH34

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q58AR3
• Locus tag: RMET_RS29840
• Protein ID: WP_011229306.1
• Coordinates: 30190..30561 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q58AR4
• Locus tag: RMET_RS29835
• Protein ID: WP_011229305.1
• Coordinates: 29996..30193 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RMET_RS29790	25239..25460	+	222	WP_011229302.1	hypothetical protein	-
RMET_RS29795	25473..25715	+	243	WP_035882428.1	hypothetical protein	-
RMET_RS29800	25712..25936	+	225	WP_011514790.1	hypothetical protein	-
RMET_RS29805	26012..26341	+	330	WP_035882430.1	hypothetical protein	-
RMET_RS29810	26373..26819	-	447	WP_011514792.1	hypothetical protein	-
RMET_RS29815	27258..27566	-	309	WP_017514069.1	hypothetical protein	-
RMET_RS29820	27855..28124	-	270	WP_124682103.1	hypothetical protein	-
RMET_RS29825	28158..29108	-	951	WP_011229304.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
RMET_RS29830	29105..29830	-	726	WP_011514795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
RMET_RS29835	29996..30193	+	198	WP_011229305.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RMET_RS29840	30190..30561	+	372	WP_011229306.1	type II toxin-antitoxin system VapC family toxin	Toxin
RMET_RS29845	30951..31169	+	219	Protein_37	hypothetical protein	-
RMET_RS33820	31855..32166	-	312	WP_049799859.1	hypothetical protein	-
RMET_RS29855	32540..32836	-	297	WP_049799860.1	hypothetical protein	-
RMET_RS33230	32898..33176	-	279	WP_011514799.1	hypothetical protein	-
RMET_RS33825	33173..33361	-	189	WP_152564866.1	hypothetical protein	-
RMET_RS29860	33438..33977	-	540	Protein_42	SAM-dependent methyltransferase	-
RMET_RS29865	34219..34668	+	450	WP_011514800.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..233720	233720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13613.83 Da
• Isoelectric Point: 5.6319

>T21566 WP_011229306.1 NC_007971:30190-30561 [Cupriavidus metallidurans CH34]
MILVDTSVWIDHLNASDPMLISLLTQERVLIHPYVIGEISLGSLRDRDVVLGALRDLPRAPVATPEETLYLIEREGLFNR
GIGYGDTSLLASARLQPGIAIWTRDKRLKKVADELCLGAVLSH

Nucleotide

• Length: 372 bp

>T21566 NC_007971:30190-30561 [Cupriavidus metallidurans CH34]
ATGATTCTTGTCGACACGTCGGTCTGGATTGACCACCTCAACGCTTCTGATCCGATGCTGATCAGCCTCTTAACCCAGGA
GCGTGTGCTGATTCATCCCTATGTGATCGGTGAGATTTCGCTTGGTAGTCTGCGGGACCGAGATGTCGTGCTTGGCGCTC
TGCGCGATTTGCCGCGAGCGCCTGTCGCAACACCAGAGGAGACCCTGTATTTGATCGAGCGCGAAGGCTTGTTCAACCGT
GGAATCGGATATGGCGATACATCATTGTTGGCATCCGCGCGTCTCCAACCAGGCATCGCCATCTGGACGCGTGACAAGCG
ATTGAAGAAAGTTGCTGATGAACTCTGTCTCGGCGCGGTCCTTTCGCACTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7132.21 Da
• Isoelectric Point: 10.3509

>AT21566 WP_011229305.1 NC_007971:29996-30193 [Cupriavidus metallidurans CH34]
MRTTIALDDELLAKAQAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIEGVPRRRQDTK

Nucleotide

• Length: 198 bp

>AT21566 NC_007971:29996-30193 [Cupriavidus metallidurans CH34]
ATGCGTACGACCATTGCGCTTGACGATGAACTGTTGGCCAAGGCCCAAGCCTATACGGGGCTGGAAGAGAAAACGGCACT
TGTGCGCGAGGCGCTAAAGGCCTTGATCCAGCGTGAAGCCGCCAAACGACTCGCGAACCTTGGCGGGAGCCAACCGGGCA
TCGAAGGGGTACCGCGTCGCCGGCAGGACACCAAATGA

Structures

Toxin

• AlphaFold DB: A0A482J4V6

Antitoxin

• AlphaFold DB: A0A482J603