Detailed information of TA system TA215643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3354361..3354981
• Replicon: chromosome
• Accession: NZ_CP082929
• Organism: Salmonella enterica subsp. enterica serovar London isolate N18-04674

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: K6991_RS16335
• Protein ID: WP_001280991.1
• Coordinates: 3354763..3354981 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: K6991_RS16330
• Protein ID: WP_000344807.1
• Coordinates: 3354361..3354735 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6991_RS16320 (3349500)	3349500..3350693	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
K6991_RS16325 (3350716)	3350716..3353865	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
K6991_RS16330 (3354361)	3354361..3354735	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
K6991_RS16335 (3354763)	3354763..3354981	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
K6991_RS16340 (3355160)	3355160..3355711	+	552	WP_023243161.1	maltose O-acetyltransferase	-
K6991_RS16345 (3355829)	3355829..3356299	+	471	WP_000136183.1	YlaC family protein	-
K6991_RS16350 (3356355)	3356355..3356495	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
K6991_RS16355 (3356501)	3356501..3356761	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
K6991_RS16360 (3356986)	3356986..3358536	+	1551	WP_000213145.1	EAL domain-containing protein	-
K6991_RS16370 (3358767)	3358767..3359156	+	390	WP_000961285.1	MGMT family protein	-
K6991_RS16375 (3359189)	3359189..3359758	-	570	WP_000779801.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T215643 WP_001280991.1 NZ_CP082929:3354763-3354981 [Salmonella enterica subsp. enterica serovar London]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T215643 NZ_CP110859:3804850-3805107 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT215643 WP_000344807.1 NZ_CP082929:3354361-3354735 [Salmonella enterica subsp. enterica serovar London]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT215643 NZ_CP110859:3805119-3805664 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0