Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) brnT-relB/BrnT-RelB
Location 24275..24615 Replicon plasmid 1
Accession NC_007968
Organism Psychrobacter cryohalolentis K5

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag PCRYO_RS13125 Protein ID WP_011512349.1
Coordinates 24275..24418 (-) Length 48 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7W2MYR8
Locus tag PCRYO_RS12880 Protein ID WP_011512369.1
Coordinates 24415..24615 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCRYO_RS12845 19461..20270 + 810 WP_011512343.1 hypothetical protein -
PCRYO_RS12850 20480..21115 + 636 WP_011512344.1 AAA family ATPase -
PCRYO_RS12855 21128..21331 + 204 WP_041753792.1 hypothetical protein -
PCRYO_RS12860 21589..22599 + 1011 WP_011512346.1 AAA family ATPase -
PCRYO_RS12865 22605..23591 + 987 WP_011512347.1 DUF4435 domain-containing protein -
PCRYO_RS12870 23751..24047 - 297 WP_041753794.1 hypothetical protein -
PCRYO_RS13125 24275..24418 - 144 WP_011512349.1 BrnT family toxin Toxin
PCRYO_RS12880 24415..24615 - 201 WP_011512369.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
PCRYO_RS12885 24717..24977 - 261 WP_011512370.1 hypothetical protein -
PCRYO_RS12890 25045..25446 - 402 WP_011512350.1 hypothetical protein -
PCRYO_RS12895 25908..26282 + 375 WP_011512351.1 hypothetical protein -
PCRYO_RS12900 26924..27871 + 948 WP_011512352.1 replication initiation protein RepM -
PCRYO_RS12905 28031..29158 + 1128 WP_011512353.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..41221 41221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-47)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-54)


Sequences

Toxin        


Download         Length: 48 a.a.        Molecular weight: 5780.34 Da        Isoelectric Point: 4.4963

>T21564 WP_011512349.1 NC_007968:c24418-24275 [Psychrobacter cryohalolentis K5]
MIEFEWDEQKNSSNQRKHGLCFEEAARVFFDPLCLRQQDRYENGEER

Download         Length: 144 bp

>T21564 NC_007968:c24418-24275 [Psychrobacter cryohalolentis K5]
ATGATTGAATTTGAATGGGATGAACAGAAAAATAGCAGTAATCAACGCAAACATGGCTTATGCTTTGAAGAAGCAGCTCG
GGTATTTTTTGATCCTTTGTGTTTGCGTCAACAAGACCGATATGAAAACGGTGAAGAGCGGTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7427.25 Da        Isoelectric Point: 4.1313

>AT21564 WP_011512369.1 NC_007968:c24615-24415 [Psychrobacter cryohalolentis K5]
MSTTNYNIRLDQELKDKAFSVLEGYGLTPSQAIKLFLHQVAETNSIPLSFDYQTMPSTNTLEDGDQ

Download         Length: 201 bp

>AT21564 NC_007968:c24615-24415 [Psychrobacter cryohalolentis K5]
ATGAGCACAACCAATTATAATATCCGTCTCGATCAAGAATTAAAAGACAAGGCGTTTTCGGTGCTTGAAGGCTATGGTTT
AACGCCTTCTCAGGCGATTAAGTTATTTTTGCATCAGGTAGCAGAAACCAATAGCATACCGCTGTCTTTTGATTACCAGA
CAATGCCTAGTACCAATACGCTTGAAGACGGTGATCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7W2MYR8

References