Detailed information of TA system TA215633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 841851..842476
• Replicon: chromosome
• Accession: NZ_CP082929
• Organism: Salmonella enterica subsp. enterica serovar London isolate N18-04674

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K6991_RS04060
• Protein ID: WP_000911337.1
• Coordinates: 842078..842476 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M7S3S8
• Locus tag: K6991_RS04055
• Protein ID: WP_000557549.1
• Coordinates: 841851..842078 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6991_RS04025 (836916)	836916..838014	+	1099	WP_010989230.1	peptide chain release factor 2	-
K6991_RS04030 (838024)	838024..839541	+	1518	WP_000003339.1	lysine--tRNA ligase	-
K6991_RS04035 (839617)	839617..840162	-	546	WP_000133994.1	isopentenyl-diphosphate Delta-isomerase	-
K6991_RS04040 (840427)	840427..841185	+	759	WP_000244328.1	amidase activator ActS	-
K6991_RS04050 (841431)	841431..841628	-	198	WP_023255488.1	hypothetical protein	-
K6991_RS04055 (841851)	841851..842078	+	228	WP_000557549.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K6991_RS04060 (842078)	842078..842476	+	399	WP_000911337.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K6991_RS04065 (843282)	843282..843818	+	537	WP_001038500.1	STM3031 family outer membrane protein	-
K6991_RS04070 (843865)	843865..844497	+	633	WP_000835264.1	YfdX family protein	-
K6991_RS04075 (845216)	845216..845800	+	585	WP_001244638.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	841851..851668	9817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15037.43 Da
• Isoelectric Point: 7.7785

>T215633 WP_000911337.1 NZ_CP082929:842078-842476 [Salmonella enterica subsp. enterica serovar London]
MLKFMLDTNTCIFTIKNKPEHIRERFNLNTSRMCISSITLMELIYGAEKSLAPERNLAVVEGFISRLEVLDYDTQAAIHT
GQIRAELARKGTPVGPYDQMIAGHARSRGLVVVTNNLREFERIPGIRIEDWC

Nucleotide

• Length: 399 bp

>T215633 NZ_CP110859:1967315-1967418 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8514.51 Da
• Isoelectric Point: 5.0210

>AT215633 WP_000557549.1 NZ_CP082929:841851-842078 [Salmonella enterica subsp. enterica serovar London]
MHTTLFFSNRTQAVRLPKSISFPEDVKHVEIIAVGRSRIITPVGESWDSWFDGEGASTDFMSTREQPAVQEREGF

Nucleotide

• Length: 228 bp

>AT215633 NZ_CP110859:c1967456-1967185 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• PDB: 6IFM
• AlphaFold DB: A0A3V2Y5V5