Detailed information of TA system TA215594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518252..518888
• Replicon: chromosome
• Accession: NZ_CP082924
• Organism: Bacillus subtilis strain FJAT-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: K8U58_RS02640
• Protein ID: WP_003156187.1
• Coordinates: 518538..518888 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: K8U58_RS02635
• Protein ID: WP_003225183.1
• Coordinates: 518252..518533 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8U58_RS02615 (514611)	514611..515210	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
K8U58_RS02620 (515305)	515305..515670	+	366	WP_003234281.1	holo-ACP synthase	-
K8U58_RS02625 (515836)	515836..516852	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
K8U58_RS02630 (516967)	516967..518136	+	1170	WP_003234284.1	alanine racemase	-
K8U58_RS02635 (518252)	518252..518533	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
K8U58_RS02640 (518538)	518538..518888	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K8U58_RS02645 (519003)	519003..519827	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
K8U58_RS02650 (519832)	519832..520197	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
K8U58_RS02655 (520201)	520201..520602	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
K8U58_RS02660 (520614)	520614..521621	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
K8U58_RS02665 (521683)	521683..522012	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
K8U58_RS02670 (522009)	522009..522491	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
K8U58_RS02675 (522457)	522457..523245	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
K8U58_RS02680 (523245)	523245..523844	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T215594 WP_003156187.1 NZ_CP082924:518538-518888 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T215594 NZ_CP110817:48580-48861 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT215594 WP_003225183.1 NZ_CP082924:518252-518533 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT215594 NZ_CP110817:48339-48590 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32