Detailed information of TA system TA215591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3812074..3812696
• Replicon: chromosome
• Accession: NZ_CP082922
• Organism: Nocardioides rotundus strain MCCC 1A10561

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K8W59_RS18745
• Protein ID: WP_223396512.1
• Coordinates: 3812301..3812696 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K8W59_RS18740
• Protein ID: WP_223396511.1
• Coordinates: 3812074..3812304 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8W59_RS18715 (K8W59_18765)	3807773..3808375	-	603	WP_223396506.1	isoprenylcysteine carboxylmethyltransferase family protein	-
K8W59_RS18720 (K8W59_18770)	3808545..3809225	-	681	WP_223396507.1	type 1 glutamine amidotransferase domain-containing protein	-
K8W59_RS18725 (K8W59_18775)	3809236..3810207	-	972	WP_223396508.1	alpha/beta hydrolase	-
K8W59_RS18730 (K8W59_18780)	3810359..3810919	+	561	WP_223396509.1	TetR-like C-terminal domain-containing protein	-
K8W59_RS18735 (K8W59_18785)	3810992..3811912	+	921	WP_223396510.1	NAD-dependent epimerase/dehydratase family protein	-
K8W59_RS18740 (K8W59_18790)	3812074..3812304	+	231	WP_223396511.1	hypothetical protein	Antitoxin
K8W59_RS18745 (K8W59_18795)	3812301..3812696	+	396	WP_223396512.1	PIN domain-containing protein	Toxin
K8W59_RS18750 (K8W59_18800)	3812700..3813653	-	954	WP_223396513.1	endonuclease/exonuclease/phosphatase family protein	-
K8W59_RS18755 (K8W59_18805)	3813870..3815087	+	1218	WP_223396514.1	sensor histidine kinase	-
K8W59_RS18760 (K8W59_18810)	3815084..3815749	+	666	WP_223396515.1	response regulator transcription factor	-
K8W59_RS18765 (K8W59_18815)	3815828..3816664	+	837	WP_223396516.1	alpha/beta hydrolase	-
K8W59_RS18770 (K8W59_18820)	3816661..3817218	+	558	WP_223396517.1	ATP-binding cassette domain-containing protein	-
K8W59_RS18775 (K8W59_18825)	3817309..3817620	+	312	WP_223396518.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3797178..3890247	93069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14083.03 Da
• Isoelectric Point: 6.2105

>T215591 WP_223396512.1 NZ_CP082922:3812301-3812696 [Nocardioides rotundus]
VSAPYLLDANALIALVVAEHEHHGRVAQWAGDVERLALCPIVEGALVRFLLRLGASGATAQALLTALHASGRCEFWEDSI
SYRSVDLDHVLGHRQVTDAYLADLAESRGARLATLDRALASARPQATYLIA

Nucleotide

• Length: 396 bp

>T215591 NZ_CP110816:c4036168-4035818 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8032.00 Da
• Isoelectric Point: 4.6684

>AT215591 WP_223396511.1 NZ_CP082922:3812074-3812304 [Nocardioides rotundus]
MRTTIDLPPAVHRRAREIARARQQSLSSVVAELATRGLNALGDPVEVGTDPVSGLPAVTLGQRVTSDDVANALDEE

Nucleotide

• Length: 231 bp

>AT215591 NZ_CP110816:c4036413-4036162 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGACGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA